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CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2022; 57(03): 488-495
DOI: 10.1055/s-0041-1741020
Artigo Original

Hancornia speciosa Gomes Latex Increases Bone Mineralization in Rats: A Preclinical Study[*]

Article in several languages: português | English
Francielly Andressa Felipetti 
1   Departamento de Morfologia, Faculdade de Odontologia de Piracicaba (FOP), Universidade Estadual de Campinas (Unicamp), Piracicaba, SP, Brasil
,
Victor Seabra Lima Prado Costa 
2   Departamento de Ciências da Saúde, Universidade Federal de Santa Catarina (UFSC), Araranguá, SC, Brasil
,
Juliana dos Santos Neves 
1   Departamento de Morfologia, Faculdade de Odontologia de Piracicaba (FOP), Universidade Estadual de Campinas (Unicamp), Piracicaba, SP, Brasil
,
Ingrid Grazielle Sousa 
1   Departamento de Morfologia, Faculdade de Odontologia de Piracicaba (FOP), Universidade Estadual de Campinas (Unicamp), Piracicaba, SP, Brasil
,
Sônia Maria De Stefano Piedade 
3   Departamento de Ciências Exatas, Escola Superior de Agricultura Luiz de Queiroz (ESALQ), Universidade de São Paulo (USP), Piracicaba, SP, Brasil
,
Pedro Duarte Novaes 
1   Departamento de Morfologia, Faculdade de Odontologia de Piracicaba (FOP), Universidade Estadual de Campinas (Unicamp), Piracicaba, SP, Brasil
› Author Affiliations Financial Support The present research was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). The project was financed through a Ph.D. scholarship and bank fees (process number 141226/2014-5), which were essential for the permanence of the students permanence and the acquisition of equipment and materials used in the research.
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Abstract

Objective To evaluate the systemic effect of Hancornia speciosa latex on bone neoformation and mineralization in rats.

Methods For that, the latex was first collected, and its composition was analyzed. A total of 30 male Wistar rats were used, which were simultaneously submitted to two surgical procedures: extraction of an incisor and creation of a defect with 2 mm in diameter in the parietal bone. The rats were divided into two groups: systemic control (SC) systemic latex (SX) which were administered, orally and daily, 1.5 mL of water or a solution containing 50% of water and 50% of latex by gavage, respectively. After 15 days of the treatment, the animals were euthanized and their samples were collected.

Results The results were statistically analyzed, and the level of significance was set at 0.05. We showed that H. speciosa latex contained calcium. The oral and daily administration of the latex for 15 days increased the contents of calcium and phosphorus in the basal bone and newly-formed bone in the mandibular alveolus of rats.

Conclusion The present was a pioneer study demonstrating the potential of H. speciosa latex in increasing bone mineralization. Our results may aid in the conception and development of a natural drug.

Keywords

apocynaceae - complementary therapies - histology - hydroxyapatites - scanning electron microscopy

* Work developed at Faculdade de Odontologia de Piracicaba (FOP), Universidade Estadual de Campinas (UNICAMP), Piracicaba, SP, Brazil.




Publication History

Received: 18 June 2021

Accepted: 09 September 2021

Article published online:
20 January 2022

© 2022. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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