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CC BY 4.0 · Glob Med Genet 2022; 09(02): 118-123
DOI: 10.1055/s-0041-1740019
Original Article

Molecular Detection of blaOXA-type Carbapenemase Genes and Antimicrobial Resistance Patterns among Clinical Isolates of Acinetobacter baumannii

Authors

  • Maghsoud Kafshnouchi

    1   Department of Microbiology, Maragheh Islamic Azad University, Maragheh, Iran

  • Marzieh Safari

    2   Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran

  • Amir Khodavirdipour 

    3   Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

  • Abbas Bahador

    4   Department of Microbiology, Tehran University of Medical Sciences, Tehran, Iran

  • Seyed Hamid Hashemi

    5   Department of Infectious Diseases, Hamadan University of Medical Sciences, Hamadan, Iran

  • Mohammad Sina Alikhani

    6   Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran

  • Massoud Saidijam

    7   Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

  • Mohammad Yousef Alikhani 

    2   Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran

Funding The present study was supported financially by the Vice-Chancellor of Research and Technology, Hamadan University of Medical Sciences, Hamadan, Iran.
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Abstract

Acinetobacter baumannii is a bacterium found in most places, especially in clinics and hospitals, and an important agent of nosocomial infections. The presence of class D enzymes such as OXA-type carbapenemases in A. baumannii is proven to have a key function in resistance to carbapenem. The aim of the current study is to determine the blaOXA-type carbapenemase genes and antimicrobial resistance among clinically isolated samples of A. baumannii. We assessed 100 clinically isolated specimens of A. baumannii from patients in intensive care units of educational hospitals of Hamadan, West of Iran. The A. baumannii isolates' susceptibility to antibiotics was performed employing disk diffusion method. Multiplex polymerase chain reaction was used to identify the blaOXA-24-like , blaOXA-23-like , blaOXA-58-like , and blaOXA-51-like genes. The blaOXA-23-like , blaOXA-24-like , and blaOXA-58-like genes' prevalence were found to be 84, 58, and 3%, respectively. The highest coexistence of the genes was for blaOXA-51/23 (84%) followed by blaOXA-51/24-like (58%). The blaOXA-51/23- like pattern of genes is a sort of dominant gene in resistance in A. baumannii from Hamadan hospitals. The highest resistance to piperacillin (83%) and ciprofloxacin (81%) has been observed in positive isolates of blaOXA-23-like . The A. baumannii isolates with blaOXA-58-like genes did not show much resistance to antibiotics. Based on the results of the phylogenetic tree analysis, all isolates have shown a high degree of similarity. This study showed the high frequency of OXA-type carbapenemase genes among A. baumannii isolates from Hamadan hospitals, Iran. Thus, applying an appropriate strategy to limit the spreading of these strains and also performing new treatment regimens are necessary.

Keywords

Acinetobacter baumannii - nosocomial infection - OXA-type carbapenemase

Consent for Publication

All authors consented for publication of the above paper.


Availability of Data and Material

All data generated or analyzed during this study are included in this published article.


Authors' Contributions

M.Y.A. designed the study, edited the manuscript. M.S. and A.B. were scientific advisor on molecular genetics section. M.K., M.S., and M.S.A. collaborated in collecting samples and doing experiments. S.H.H. collaborated on patient introduction and clinical examination. A.K. wrote the scientific draft and checked the English use and grammar of the manuscript.




Publikationsverlauf

Eingereicht: 20. September 2021

Angenommen: 18. Oktober 2021

Artikel online veröffentlicht:
02. Dezember 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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