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CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2022; 57(06): 992-1000
DOI: 10.1055/s-0041-1739174
Artigo Original
Ombro e Cotovelo

Automated Assessment of Cell Infiltration and Removal in Decellularized Scaffolds – Experimental Study in Rabbits

Article in several languages: português | English
Alex de Lima Santos 
1   Departamento de Ortopedia e Traumatologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
,
Camila Gonzaga da Silva 
2   Departamento de Cirurgia, Escola Paulista de Medicina, Universidade Federal de São Paulo, SP, Brasil
,
Leticia Siqueira de Sá Barreto 
2   Departamento de Cirurgia, Escola Paulista de Medicina, Universidade Federal de São Paulo, SP, Brasil
,
Marcel Jun Sugawara Tamaoki 
1   Departamento de Ortopedia e Traumatologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
,
Fernando Gonçalves de Almeida 
2   Departamento de Cirurgia, Escola Paulista de Medicina, Universidade Federal de São Paulo, SP, Brasil
,
Flavio Faloppa 
1   Departamento de Ortopedia e Traumatologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
› Author Affiliations Financing Support The present study was funded by the National Council for Scientific and Technological Development (CNPq) – process number 311237/2018-5.
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Abstract

Objective Semiquantitative and automated measurement of nuclear material removal and cell infiltration in decellularized tendon scaffolds (DTSs).

Method 16 pure New Zealand rabbits were used, and the gastrocnemius muscle tendon was collected bilaterally from half of these animals (16 tendons collected); 4 were kept as control and 12 were submitted to the decellularization protocol (DTS). Eight of the DTSs were used as an in vivo implant in the experimental rotator cuff tear (RCT) model, and the rest, as well as the controls, were used in the semiquantitative and automated evaluation of nuclear material removal. The eight additional rabbits were used to make the experimental model of RCT and subsequent evaluation of cellular infiltration after 2 or 8 weeks, within the DTS.

Results The semiquantitative and automated analysis used demonstrated a removal of 79% of nuclear material (p < 0.001 and power > 99%) and a decrease of 88% (p < 0.001 and power >99%) in the area occupied by nuclear material after the decellularization protocol. On cell infiltration in DTS, an increase of 256% (p < 0.001 and power >99%) in the number of cells within the DTS was observed in the comparison between 2 and 8 weeks postoperatively.

Conclusion The proposed semiquantitative and automated measurement method was able to objectively measure the removal of nuclear material and cell infiltration in DTS.

Keywords

tendons - tissue engineering - tissue scaffolds - extracellular matrix - regenerative medicine

Study developed at Escola Paulista de Medicina da Universidade Federal de São Paulo, São Paulo, SP, Brazil




Publication History

Received: 30 October 2020

Accepted: 25 June 2021

Article published online:
20 December 2021

© 2021. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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