Chemical and pharmacological comparison of two chemotypes of Psidium cattleyanum: a rational approach for marker selection

Psydium cattleyanum (PC; araçá) leaves are popularly used in Brazil for their anti-inflammatory properties. However, there are two types of PC: one which presents fruits with yellow epicarp and the one with red epicarp. This rises a question if the leaves of both types can be used interchangeable. To address this issue, 28 samples of the leaves of PC were obtained and extracted using the same method assisted by ultrasound. All extracts were analyzed by HPLC-DAD and the chromatograms were converted to the matrix X. The data were aligned and further transformed by column centering. This new matrix was analyzed by principal component analysis (PCA). Two chemotypes were detected; however, not related to fruit color. To identify which compounds could be responsible for this segregation, the loading plot of PC1 (54% of the variation) was compared to the chromatograms.

Further UHPLC-Q-TOF-MS analysis, allowed to identify quercetin xylopyranoside and quercetin arabinoside as the main compounds responsible for the separation of a second chemotype. On the other hand, miquelianin (1), quercitrin (2), and hyperoside (3) were detected for both chemotypes. One representative sample of each chemotype, as well as 1-3 were evaluated in vitro for their anti-chemotactic activity, as an indirect measure of their anti-inflammatory potential. Both extracts and the isolated compounds exhibited similar IC₅₀ (0.5 μg/mL). Taken together, these results indicate that both chemotypes can be used interchangeable and that 1-3 can be used as bioactive markers for the quality control of PC.

Publikationsverlauf

Artikel online veröffentlicht:
13. Dezember 2021