Quantification of Citrus limon (L.) Burmin in complete feed with UHPLC-MS/MS using a double “one-point” standard addition

A specific and sensitive method for quantification of Citrus limon (L.) Burmin in complete feed based on UHPLC-MS/MS has been developed and validated. The additive studied is a natural commercial feed additive (Citrozest®, Nor-Feed, France) used in animal nutrition, consisting of a standardized extract of Citrus limon (L.) Burmin in which eriocitrin has been identified and quantified. Eriocitrin was used as phytomarker for the quantification of the additive with a level of 67 ng/g of phytomarker in complete feed. Eriocitrin quantification is performed by the standard addition method prior HPLC-MS/MS analysis. To compensate for the loss of eriocitrin during the extraction step, cameliaside A is used as internal standard and the recovery is also determined by standard addition method. Standard addition method may be too much time consuming for routine analysis. To decrease the workload of the analysis, a simplified approach based on the “one-point” addition was used for both standard additions permitting the utilization of the method in routine. The method was developed and validated in-house in accordance with the guidelines recommended by IUPAC [1] for the quantification of Citrus limon (L.) Burmin in complete feed. This method of analysis has all the prerequisites to be used by European authorities as part of the registration of a feed additive [2].

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Artikel online veröffentlicht:
13. Dezember 2021

• References

• 1 Thompson M, Ellison SLR, Wood R. Pure Appl Chem 2002; 74: 835–855
• 2 FEEDAP. EFSA J 2012; 10: 2536