Planta Med 2021; 87(15): 1248
DOI: 10.1055/s-0041-1736775
Abstracts
3. Short Lectures

Isolation of cytotoxic compounds from the underground parts of Valeriana sisymbriifolia Vahl. through activity-guided fractionation

M Erdoğan
1   Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, İstanbul, Turkey
,
B Aru
2   Department of Immunology, Faculty of Medicine, Yeditepe University, İstanbul, Turkey
,
ŞT Yılmaz
3   Faculty of Pharmacy, Yeditepe University, İstanbul, Turkey
,
E Yeşilada
1   Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, İstanbul, Turkey
,
G Yanıkkaya Demirel
2   Department of Immunology, Faculty of Medicine, Yeditepe University, İstanbul, Turkey
,
H Kırmızıbekmez
1   Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, İstanbul, Turkey
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Valeriana sisymbriifolia is a perennial herb that is particularly distributed in Turkey, Iran and Iraq [1]. Previous studies on Valeriana species indicated sedative, anxiolytic, neuroprotective and cytotoxic activities which are mainly attributed to its iridoids and sesquiterpenes [2]. In this study, we aimed to isolate the cytotoxic compounds from underground parts of V. sisymbriifolia through in vitro activity-guided fractionation technique. The EtOH extract, its fractions and isolates were evaluated for their cytotoxic activities against four cancer cell lines (MCF-7, HGC-27, PC-3, A549) and against HUVEC cell line by MTS assay. The CHCl3 and EtOAc fractions exhibited remarkable cytotoxic activity against the tested cancer cell lines (IC50: 2.4-16.3 µg/mL). Thus, CHCl3 and EtOAc fractions were separated by chromatographic methods to give two new iridoids (1 and 2) and two new sesquiterpene lactones (3 and 4) together with nine known compounds, didrovaltrate (5), valtrate (6), 7-homovaltrate (7), 1-α-acevaltrate (8), baldrinal (9), 11-ethoxyviburtinal (10), patriscabrol (11), bornyl caffeate (12) and caproleic acid (13). The structures of the isolates were elucidated by NMR and HR-MS analyses. 6 showed strong cytotoxic activity on A549 cell line with IC50 value of 7.5 µM while 5, 6, 7, and 8 exhibited significant cytotoxic activities on MCF-7 cell line with IC50 values ranging from 2.5 to 5.9 µM. Also, 6 and 7 indicated the best cytotoxic activities against HGC-27 cell line (IC50: 2.3 and 3.7 µM respectively). 6, 7 and 8 showed noteworthy cytotoxic activity against PC-3 (IC50: 2.3-9.7 µM). The mechanisms behind the cytotoxic activities are underway.

Acknowledgements This study was supported by TÜBİTAK (Project No: 119Z094)

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Publication History

Article published online:
13 December 2021

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