Innovative Approaches to African Traditional Medicine in analysis of purity, quality and potency of new Phytopharmaceutical extracts.

A proposed HPTLC technique for Kigelia pinnata fruit extract. Commercial extracts of Kigelia pinnata HAB19F 30% EtOh was obtained from suppliers Company 1. and by commercial supplier company 2. Using CAMAG HPTLC Automatic Sampler with HPTLC glass 20x10 cm, Si 60 F254 using solvent system of Ethanol, Chloroform and Ethyl Acetate, developed with 0.5% anisaldehyde reagent in methanol: acetic acid: sulphuric acid (85:10:5). For purposes of this experiment a known compound found in Kigelia, β-Bisobolol was used as a reference standard, the results indicate difference in quality of extracts and purity from different commercial companies it also indicates that further chromatographic analysis needs to be undertaken to show better separation of active ingredients and identify a better reference compound. These results show that further monograph profiling work and HPTLC analysis needs to be undertaken before standardised commercial extracts and a standardised monograph can occur on extracts of Kigelia pinnata.

Publication History

Article published online:
13 December 2021