CC BY-NC-ND 4.0 · J Lab Physicians 2022; 14(01): 006-010
DOI: 10.1055/s-0041-1732809
Original Article

Molecular Characterization Identifies Upstream Presence of ISAba1 to OXA Carbapenemase Genes in Carbapenem-Resistant Acinetobacter baumannii

Nageswari Gandham
1   Department of Microbiology, Dr. D.Y. Patil Medical College, Hospital and Research Center (Dr. D.Y. Patil Vidyapeeth, Pune) Pimpri, Maharashtra, India
,
Neetu Gupta
1   Department of Microbiology, Dr. D.Y. Patil Medical College, Hospital and Research Center (Dr. D.Y. Patil Vidyapeeth, Pune) Pimpri, Maharashtra, India
,
Chanda Vyawahare
1   Department of Microbiology, Dr. D.Y. Patil Medical College, Hospital and Research Center (Dr. D.Y. Patil Vidyapeeth, Pune) Pimpri, Maharashtra, India
,
Shahzad Beg Mirza
1   Department of Microbiology, Dr. D.Y. Patil Medical College, Hospital and Research Center (Dr. D.Y. Patil Vidyapeeth, Pune) Pimpri, Maharashtra, India
,
Rabindra Nath Misra
1   Department of Microbiology, Dr. D.Y. Patil Medical College, Hospital and Research Center (Dr. D.Y. Patil Vidyapeeth, Pune) Pimpri, Maharashtra, India
› Author Affiliations

Abstract

Background Evaluating the expression pattern of oxacillinases (OXA) carbapenemases is essential to understand the prevalence and spread of carbapenem resistance Acinetobacter baumannii.

Objectives The aim of the study is to evaluate the presence of OXA carbapenemase genes and ISAba1 upstream to these genes in carbapenem-resistant A. baumannii clinical isolates.

Materials and MethodsA. baumannii isolated from clinical samples were phenotypically identified and antibiotics sensitivity was performed. Multiplex polymerase chain reaction (PCR) was used to detect OXA51-like gene, OXA carbapenemases genes (OXA-23-like, OXA-24-like, and OXA-58-like), and ISAba1 in carbapenem-resistant isolates.

Results Out of 55 Acinetobacter isolates, 54 were confirmed as A. baumannii by PCR. BlaOXA-23-like gene was observed in 51 isolates of A. baumannii and none of the isolates showed the presence of blaOXA-24-like and blaOXA-58-like genes. Presence of ISAba1 upstream to OXA-23-like gene, OXA-51-like gene, and both OXA-51-like/OXA-23-like genes was observed in 51, 7, and 4 A. baumannii isolates, respectively.

Conclusion The genetic pattern of carbapenem-resistant A. baumannii isolated in this study was unique, which should be factored for clinical protocols to manage infections caused by emerging resistant strains of A. baumannii.

Supplementary Material



Publication History

Article published online:
31 August 2021

© 2021. The Indian Association of Laboratory Physicians. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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