CC BY-NC-ND 4.0 · J Lab Physicians 2022; 14(02): 132-138
DOI: 10.1055/s-0041-1732808
Original Article

Evaluation of Erythrocytes Magnetized Technology for Measurement of ABO Isoagglutinin Titers

Parul Chopra
1   Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India
Sunanda Bhardwaj
2   Department of Hematology and Immunology, Dr. Lal PathLabs Ltd., National Reference Lab, New Delhi, India
Ajay Samkaria
3   DIAGAST, India
Asha Amoli
2   Department of Hematology and Immunology, Dr. Lal PathLabs Ltd., National Reference Lab, New Delhi, India
Anil Arora
2   Department of Hematology and Immunology, Dr. Lal PathLabs Ltd., National Reference Lab, New Delhi, India
› Author Affiliations


Background A variation in the measurement of ABO antibody titer has been seen among different laboratories due to lack of standardization. In our study, we aim to evaluate automated ABO isoagglutinin titer measurements by erythrocytes magnetized technology (EMT) and compare with conventional tube technique (TT).

Methods We performed ABO isoagglutinin titration on samples received in a reference laboratory during a period of 2 months. A total of 134 tests for immunoglobulin G (IgG) titer and 116 for immunoglobulin M (IgM) for anti-A or anti-B were included in the study. Samples were processed for ABO isoagglutination titers by both TT and EMT by QWALYS-3 (DIAGAST, France). Microsoft Excel was used to compile data, for all calculations, and to draw graphs and plots. The number and percentage of cases within ±1, ±2, or ±3 titer difference (TT-EMT) were calculated.

Results Median titers and their ranges obtained by EMT were higher or equal to those by TT for all IgM and IgG ABO-antibodies in all blood group (BGs), except anti-A IgM in (BG) O that was lower by EMT (32 [4:128]) than TT (48 [8:256]). One twenty one (121/134, 90.3%) cases of IgG titer showed an agreement by both methods (within ± one titer difference). One hundred seven cases (107/116, 92.2%) for IgM titer were within one titer difference by both the methods.

Conclusion Results of titration by EMT-based automated instrument QWALYS-3 and conventional TT may vary by one titer dilution in the majority of cases. Use of consistent method for patient management is, therefore, advised.

Authors’ Contribution

Parul Chopra was involved in definition of intellectual content, literature search, interpretation of results, data analysis, and drafting and editing of the manuscript. Sunanda Bhardwaj interpreted results and edited the manuscript. Ajay Samkaria carried out the tests in the laboratory, analyzed the data, and edited the manuscript. Asha Amoli carried out the tests in the laboratory, interpreted the results, did data entry, and edited the manuscript. Anil Arora conceptualized the study, defined intellectual content interpretation of results, and edited the manuscript.

Ethical Approval

Since the tests were done on residual samples obtained in the laboratory for testing, ethical clearance was not required.

Publication History

Article published online:
15 July 2021

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