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CC BY-NC-ND 4.0 · J Lab Physicians 2021; 13(04): 358-361
DOI: 10.1055/s-0041-1732494
Original Article

Evaluation of HiCrome KPC Agar for the Screening of Carbapenem-Resistant Enterobacterales Colonization in the ICU Setting of a Tertiary Care Hospital

Ashoka Mahapatra 
1   Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Orissa, India
,
K Nikitha 
2   All India Institute of Medical Sciences, Bhubaneswar, Orissa, India
,
Sutapa Rath 
1   Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Orissa, India
,
Bijayini Behera 
1   Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Orissa, India
,
Kavita Gupta 
1   Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Orissa, India
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Abstract

Background Spread of carbapenem-resistant Enterobacterales (CRE) is a significant concern in intensive care unit (ICU) settings. Approaches to routine screening for CRE colonization in all ICU patients vary depending on institutional epidemiology and resources. The present study was aimed to evaluate the performance of HiCrome Klebsiella pneumoniae carbapenemase (KPC) agar for the detection of CRE colonization in ICU settings taking the Centers for Disease Control and Prevention (CDC) recommended method as reference.

Methods Two-hundred and eighty rectal swabs (duplicate) from 140 patients were subjected to CRE detection in HiCrome KPC agar and MacConkey agar (CDC criteria).

Results Using CDC method, total 41 CRE isolates were recovered comprising of 29 Escherichia coli, 11 Klebsiella, and 1 Enterobacter spp. On the other hand, 49 isolates of CRE recovered from 140 rectal swabs using HiCrome KPC agar, out of which 33 were E. coli, 15 Klebsiella, and 1 Enterobacter sp.

Statistical Analysis Sensitivity, specificity, negative, and positive predictive values of CRE screening by HiCrome KPC agar were found to be 100% (91.4–100), 91.9% (84.8–95.8), 83.6% (70.9–91.4), and 100% (95.9–100), respectively, taking the CDC recommended method as reference.

Conclusion HiCrome KPC agar has high sensitivity in screening CRE colonization. Further studies are needed to establish its applicability for detecting the predominant circulating carbapenemases in the Indian setting.

Keywords

CRE - HiCrome KPC agar - rectal swab


Publication History

Article published online:
12 July 2021

© 2021. The Indian Association of Laboratory Physicians. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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