CC BY-NC-ND 4.0 · Revista Urología Colombiana / Colombian Urology Journal 2021; 30(03): e194-e198
DOI: 10.1055/s-0041-1731413
Original Articles | Artículos Originales
General urology / Urología General

The Effect of Glyphosate on Human Sperm: In Vitro Approximation

Efecto del glifosato sobre los espermatozoides humanos: una aproximación in vitro
Mateo Morales Velásquez
1   Reproduction Group, Department of Microbiology and Parasitology, School of Medicine, Universidad de Antioquia, Medellín, Colombia
Valentina Velásquez Rivera
1   Reproduction Group, Department of Microbiology and Parasitology, School of Medicine, Universidad de Antioquia, Medellín, Colombia
Walter D. Cardona Maya
1   Reproduction Group, Department of Microbiology and Parasitology, School of Medicine, Universidad de Antioquia, Medellín, Colombia
› Author Affiliations


Introduction Glyphosate is an herbicide used to eradicate illicit crops; however, its use is controversial due to different health problems associated with it. The present study aims to evaluate the effects of glyphosate on human sperm in vitro.

Methods Twenty-two semen samples from healthy normozoospermic men were included; 11 semen samples were incubated with Panzer (INVESA S.A., Antiquia, Colombia) and 11 with Roundup (Monsanto Company, MO, USA). The changes in motility and viability were observed. Functional seminal parameters were evaluated as well.

Results The samples exposed to glyphosate showed less motility and viability; a decrease in the potential of the mitochondrial membrane was observed, and an increase in the lipoperoxidation of the membrane was evidenced.

Conclusion Based on the present results, we concluded that glyphosate has cytotoxic potential for exposed people and may affect their fertility.


Introducción El glifosato es un herbicida utilizado ampliamente para la erradicación de cultivos ilícitos; sin embargo, su uso es polémico debido a diferentes problemas de salud asociados con él. El objetivo del presente estudio fue evaluar los efectos del glifosato sobre los espermatozoides humanos in vitro.

Métodos Se incluyeron 22 muestras de semen de hombres sanos normozoospérmicos, de las cuales 11 se incubaron con Panzer y 11 con Roundup, y se evaluaron los cambios en la movilidad y la viabilidad espermática, además de valorar los parámetros seminales funcionales.

Resultados Las muestras expuestas al glifosato presentaron una menor movilidad y viabilidad, una disminución en el potencial de la membrana mitocondrial, y un aumento en la lipoperoxidación de la membrana.

Conclusiones El glifosato es potencialmente citotóxico para las personas que estén expuestas, y puede afectar su fertilidad.

Publication History

Received: 21 January 2021

Accepted: 10 March 2021

Article published online:
30 September 2021

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