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High-Frequency Three-Dimensional Lumen Volume Ultrasound Is a Sensitive Method to Detect Early Aneurysmal Change in Elastase-Induced Murine Abdominal Aortic AneurysmFunding This work was funded by the British Heart Foundation (BHF). M.A.W. is a BHF clinical training research fellow and M.A.B. is a BHF intermediate clinical fellow.
Objective The aim of this study was to investigate the reproducibility of anterior–posterior diameter (APdmax) and three-dimensional lumen volume (3DLV) measurements of abdominal aortic aneurysms (AAA) in a classical murine AAA model. We also compared the magnitude of change in the aortic size detected with each method of assessment.
Methods Periadventitial application of porcine pancreatic elastase (PPE AAA) or sham surgery was performed in two cohorts of mice. Cohort 1 was used to assess for observer variability with the APdmax and 3DLV measurements. Cohort 2 highlighted the relationship between APdmax and 3DLV and changes in AAA detected.
Results There was no significant observer variability detected with APdmax measurement. Similarly, no significant intraobserver variability was evident with 3DLV; however, a small but significant interobserver difference was present. APdmax and 3DLV measurements of PPE AAA significantly correlated. However, changes in the AAA morphology were detected earlier with 3DLV.
Conclusion APdmax and 3DLV are both reliable methods for measuring an AAA. Both these methods correlate with each other. However, changes in AAA morphology were detected earlier with 3DLV, which is important to detect subtle but important changes to aortic geometry in a laboratory setting. 3DLV measurement of AAA is a simple, reproducible, and comprehensive method for assessing changes in disease morphology.
Received: 12 October 2020
Accepted: 21 March 2021
Article published online:
28 December 2021
© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
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- 1 Ashton HA, Buxton MJ, Day NE. et al; Multicentre Aneurysm Screening Study Group. The Multicentre Aneurysm Screening Study (MASS) into the effect of abdominal aortic aneurysm screening on mortality in men: a randomised controlled trial. Lancet 2002; 360 (9345): 1531-1539
- 2 Bhamidipati CM, Mehta GS, Lu G. et al. Development of a novel murine model of aortic aneurysms using peri-adventitial elastase. Surgery 2012; 152 (02) 238-246
- 3 Guo X, Kono Y, Mattrey R, Kassab GS. Morphometry and strain distribution of the C57BL/6 mouse aorta. Am J Physiol Heart Circ Physiol 2002; 283 (05) H1829-H1837
- 4 Martin-McNulty B, Vincelette J, Vergona R, Sullivan ME, Wang Y-X. Noninvasive measurement of abdominal aortic aneurysms in intact mice by a high-frequency ultrasound imaging system. Ultrasound Med Biol 2005; 31 (06) 745-749
- 5 Barisione C, Charnigo R, Howatt DA, Moorleghen JJ, Rateri DL, Daugherty A. Rapid dilation of the abdominal aorta during infusion of angiotensin II detected by noninvasive high-frequency ultrasonography. J Vasc Surg 2006; 44 (02) 372-376
- 6 Ghulam QM, Kilaru S, Ou S-S, Sillesen H. Clinical validation of three-dimensional ultrasound for abdominal aortic aneurysm. J Vasc Surg 2020; 71 (01) 180-188
- 7 Long A, Rouet L, Debreuve A. et al. Abdominal aortic aneurysm imaging with 3-D ultrasound: 3-D-based maximum diameter measurement and volume quantification. Ultrasound Med Biol 2013; 39 (08) 1325-1336
- 8 Goldberg A, Pakkiri P, Dai E, Lucas A, Fenster A. Measurements of aneurysm morphology determined by 3-d micro-ultrasound imaging as potential quantitative biomarkers in a mouse aneurysm model. Ultrasound Med Biol 2007; 33 (10) 1552-1560
- 9 Sawada H, Chen JZ, Wright BC, Moorleghen JJ, Lu HS, Daugherty A. Ultrasound imaging of the thoracic and abdominal aorta in mice to determine aneurysm dimensions. J Vis Exp 2019;145
- 10 Cao RY, Amand T, Ford MD, Piomelli U, Funk CD. The murine angiotensin II-induced abdominal aortic aneurysm model: rupture risk and inflammatory progression patterns. Front Pharmacol 2010; 9: 1-9
- 11 Soepriatna AH, Damen FW, Vlachos PP, Goergen CJ. Cardiac and respiratory-gated volumetric murine ultrasound. Int J Cardiovasc Imaging 2018; 34 (05) 713-724
- 12 Jansen CHP, Reimann C, Brangsch J, Botnar RM, Makowski MR. In vivo MR-angiography for the assessment of aortic aneurysms in an experimental mouse model on a clinical MRI scanner: comparison with high-frequency ultrasound and histology. PLoS One 2017; 12 (06) e0178682
- 13 Trachet B, Fraga-Silva RA, Piersigilli A. et al. Dissecting abdominal aortic aneurysm in Ang II-infused mice: suprarenal branch ruptures and apparent luminal dilatation. Cardiovasc Res 2015; 105 (02) 213-222
- 14 Daugherty A, Cassis LA. Mouse models of abdominal aortic aneurysms. Arterioscler Thromb Vasc Biol 2004; 24 (03) 429-434
- 15 Powell JT, Sweeting MJ, Ulug P. et al; EVAR-1, DREAM, OVER and ACE Trialists. Meta-analysis of individual-patient data from EVAR-1, DREAM, OVER and ACE trials comparing outcomes of endovascular or open repair for abdominal aortic aneurysm over 5 years. Br J Surg 2017; 104 (03) 166-178
- 16 Daugherty A, Manning MW, Cassis LA. Angiotensin II promotes atherosclerotic lesions and aneurysms in apolipoprotein E-deficient mice. J Clin Invest 2000; 105 (11) 1605-1612