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CC BY 4.0 · Pharmaceutical Fronts 2021; 03(01): e30-e37
DOI: 10.1055/s-0041-1730985
Original Article

The Expression of Recombinant Human Serum Albumin in the Mammary Gland of Transgenic Mice

Gui-Hua Gong
1   Biopharmaceutical Department, China State Institute of Pharmaceutical Industry, Shanghai, People's Republic of China
,
Shu Han
1   Biopharmaceutical Department, China State Institute of Pharmaceutical Industry, Shanghai, People's Republic of China
,
Xiao-Ling Huang
1   Biopharmaceutical Department, China State Institute of Pharmaceutical Industry, Shanghai, People's Republic of China
,
Li-Ping Xie
1   Biopharmaceutical Department, China State Institute of Pharmaceutical Industry, Shanghai, People's Republic of China
,
Wei Zhang
1   Biopharmaceutical Department, China State Institute of Pharmaceutical Industry, Shanghai, People's Republic of China
,
Lei Xu
1   Biopharmaceutical Department, China State Institute of Pharmaceutical Industry, Shanghai, People's Republic of China
,
You-Jia Hu
1   Biopharmaceutical Department, China State Institute of Pharmaceutical Industry, Shanghai, People's Republic of China
› Institutsangaben
Funding This work was supported by Sinopharm New Product Development Foundation (Grant No. 2013SW28).
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Abstract

Human serum albumin (HSA) is widely used in the clinic for the treatment of several diseases in large amount each year. With the increasing demands of HSA in clinic and limited blood resource, recombinant HSA (rHSA) is becoming an attractive and alternative source for HSA production. In this study, we aimed to express rHSA in the mammary glands of transgenic mice by using a tissue-specific promoter and other regulatory elements. An rHSA expression vector was constructed bearing the cDNA and first intron of HSA under the control of bovine αs1-casein promoter with a 2 × chicken β-globin insulator in the front. Transgenic mice were generated and reverse transcription polymerase chain reaction showed that rHSA was expressed only in the mammary gland, indicating the tissue specificity of the bovine αs1-casein promoter in directing transgene transcription in transgenic mice. Enzyme-linked immunosorbent assay test showed that rHSA was successfully secreted into the milk of transgenic mice with the highest level at 1.98 ± 0.12 g/L. Our results indicate the ability of the bovine αs1-casein promoter to induce successful expression of rHSA in the mammary gland of transgenic mice.

Keywords

transgenic mice - mammary gland - recombinant human serum albumin


Publikationsverlauf

Eingereicht: 23. März 2021

Angenommen: 09. April 2021

Artikel online veröffentlicht:
23. Juni 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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