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CC BY-NC-ND 4.0 · Journal of Coloproctology 2021; 41(02): 138-144
DOI: 10.1055/s-0041-1730258
Original Article | Artigo Original

Antioxidant Evaluation of the Aqueous Extract of Hulls of Campsiandra laurifolia in Colitis Induced by Acetic Acid in Wistar Rats

Avaliação antioxidante do extrato aquoso de cascas de Campsiandra laurifolia na colite induzida por ácido acético em ratos Wistar
Lucas Petitemberte de Souza 
1   Universidade Luterana do Brasil, Canoas, RS, Brasil
,
Henrique Sarubbi Fillmann 
2   Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brasil
,
Sandielly Rebeca Benitez da Fonseca 
3   Programa de Pós-Graduação em Ciências Médicas; Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil
,
Marilda da Silva Brasil 
4   Programa de Pós-Graduação em Ciências Biológicas: Fisiologia; Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil
,
Norma Anair Possa Marroni 
5   Departamento de Fisiologia; Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil
,
Alexandre de Barros Falcão Ferraz 
6   Laboratory of Phytochemistry, Universidade Luterana do Brasil, Canoas, RS, Brasil
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Abstract

Due to the ethnopharmacological use of Campsiandra laurifolia (Fabaceae), popularly known as Acapurana, to treat wounds and ulcers, associated with the lack of alternative treatments for intestinal inflammations such as ulcerative colitis (UC), the present work sought to characterize its phytochemical and antioxidant activities, and to evaluate remedial action in experimental colitis with acetic acid. Phytochemical analyzes were performed through qualitative and quantitative colorimetric tests of the main secondary metabolites. In the colitis model, 24 male Wistar rats aged ± 60 days old were used, divided into 4 groups: Control (CO) control + aqueous extract of C. laurifolia 50 mg/kg (CO + A50); Colitis (CL); and Colitis + aqueous extract of C. laurifolia 50 mg/kg (CL + A50). Measurement of sphincter anal pressure and histological tests of the large intestine, lipoperoxidation (LPO), enzyme activity of superoxide dismutase (SOD), and levels of glutathione (GSH) were performed. For statistical analysis, the oxidative stress (OS) results were expressed as means ± standard error, adopting a significance level of p < 0.05. The screening indicated the presence of flavonoids, saponins and tannins in the extract, with high levels of phenolic compounds and tannins, and was related to high antioxidant capacity. In the histological analysis, the CL group presented loss of the crypts, edema and inflammatory infiltrate. The use of C. laurifolia extract restructured the crypts, decreased edema and increased sphincter anal pressure, with a decrease in LPO, SOD, and an increase in GSH. It is suggested that the use of C. laurifolia extract reduces OS due to its antioxidant power conferred by the phenolic compounds present in the extract.

Resumo

Devido ao uso etnofarmacológico de Campsiandra laurifolia (Fabaceae), popularmente conhecida como Acapurana, para tratar feridas e úlceras, associado à falta de alternativas de tratamentos para as inflamações intestinais como a retocolite ulcerativa (RCU), o presente trabalho buscou caracterizar sua constituição fitoquímica, sua atividade antioxidante, e avaliar sua ação reparadora na colite experimental com ácido acético. As análises fitoquímicas foram realizadas por meio de ensaios colorimétricos qualitativos e quantitativos dos principais metabólitos secundários. No modelo de colite, foram utilizados 24 ratos machos Wistar de ± 60 dias de idade, divididos em 4 grupos: Controle (CO), controle + extrato aquoso de C. laurifolia 50 mg/kg (CO + A50); Colite (CL); e Colite + extrato aquoso de C. laurifolia (CL + A50). Foram realizadas aferições da pressão anal esfincteriana e avaliações histológicas do intestino grosso, lipoperoxidação (LPO), atividade da enzima superóxido dismutase (SOD) e níveis da glutationa (GSH). Para a análise estatística, resultados do estresse oxidativo (EO) foram expressos em médias ± erro padrão, adotando um nível de significância de p < 0,05. O screening indicou no extrato a presença de flavonoides, saponinas e taninos com altos teores de compostos fenólicos e taninos, relacionando-os a uma elevada capacidade antioxidante. Na análise histológica, o grupo CL apresentou perda das criptas, do edema e do infiltrado inflamatório. O uso do extrato de C. laurifolia reestruturou as criptas, diminuiu o edema e aumentou a pressão anal esfincteriana, com diminuição da LPO, da SOD, e aumento da GSH. Sugere-se que o uso do extrato de C. laurifolia diminui o EO por seu poder antioxidante, conferido pelos compostos fenólicos presentes no extrato.

Keywords

antioxidant - tannins - oxidative stress - colitis

Palavras-chave

antioxidante - taninos - estresse oxidativo - colite

Sources of Support

CAPES; CNPq; FIPE-HCPA




Publikationsverlauf

Eingereicht: 22. Mai 2020

Angenommen: 15. Januar 2021

Artikel online veröffentlicht:
30. Juni 2021

© 2021. Sociedade Brasileira de Coloproctologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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