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CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2022; 12(01): 67-70
DOI: 10.1055/s-0041-1730107
Original Article

Age-Related Effects on Right Femoral Bone of Male Wistar Rats: A Morphometric and Biomechanical Study

Sheila Martins Puelker
1   Department of Aging Sciences, Sao Judas Tadeu University, Rua Taquari, Sao Paulo, Brazil
,
Sonia Regina Ribeiro de Castro 
1   Department of Aging Sciences, Sao Judas Tadeu University, Rua Taquari, Sao Paulo, Brazil
,
Romeu Rodrigues de Souza
2   Department of Anatomy, Nove de Julho University, Rua Vergueiro, Sao Paulo, Brazil
,
Laura Beatriz Mesiano Maifrino
3   Medical School of the ABC District, Av. Lauro Gomes, Sao Paulo, Brazil
4   Dante Pazzanese Institute of Cardiology, Av. Dr. Dante Pazzanese, Sao Paulo, Brazil
,
Ricardo Aparecido Baptista Nucci
1   Department of Aging Sciences, Sao Judas Tadeu University, Rua Taquari, Sao Paulo, Brazil
5   Department of Pathology, University of Sao Paulo Medical School, Sao Paulo, Brazil
,
Maria do Carmo Sitta
6   Division of Geriatrics, University of Sao Paulo Medical School, Sao Paulo, Brazil
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Abstract

Introduction Study of the variations of bone characteristics with age in different animal models is important to design musculoskeletal studies. Thus, this study aimed to evaluate the bone mass, dimensions, and biomechanical parameters of the femur in young, middle-aged, and aged Wistar rats.

Materials and Methods Thirty male rats (Rattus norvegicus) were divided in three groups (n = 10 per group)—3-month-old young rats, 12-month-old middle-aged rats, and 18-months-old aged rats. The right femurs were subjected sequentially to morphometric study (bone weight, cortical thickness) and biomechanical tests (maximum resistance strength and bone stiffness).

Results We observed a significant increase in femur histological (cortical thickness) and biomechanical (maximum strength and bone stiffness) parameters with aging when compared with young animals.

Conclusions With the advancing age, the right femoral bone of middle-aged and old animals had greater variations when compared with young animals. However, further studies with the aid of a comparison between right and left femur and other long bones in both male and female rats are needed to corroborate with our findings.

Keywords

aging - biomechanics - bone


Publication History

Article published online:
01 June 2021

© 2021. Nitte (Deemed to be University). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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