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CC BY-NC-ND 4.0 · J Pediatr Infect Dis 2021; 16(04): 171-177
DOI: 10.1055/s-0041-1728743
Original Article

Cytomegalovirus Genotype and Virulence in Infants with Congenital Infection

Hong-bo Hu
1   Department of Laboratory, Maternal and Child Health Hospital of Hubei Province, People's Republic of China
,
Jian-gang Wu
2   Department of Laboratory, First People's Hospital of Guangshui, People's Republic of China
,
Jian-jun Sun
2   Department of Laboratory, First People's Hospital of Guangshui, People's Republic of China
,
Qiao-ying Peng
3   Department of Neonatology, Maternal and Child Health Hospital of Hubei Province, People's Republic of China
,
Xiao-peng Shang 
4   Department of Infectious Disease, First People's Hospital of Guangshui, People's Republic of China
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Abstract

Objective Cytomegalovirus (CMV) virulence may depend on genetic variability in several regions of the genome. This study aimed to assess specific CMV genotypes' association with the severity of symptomatic congenital CMV disease at birth.

Methods CMV glycoprotein B (gB), glycoprotein N (gN), glycoprotein H (gH), and UL144 strains were identified by nested polymerase chain reaction, restriction fragment length polymorphism, and heteroduplex mobility assay single-stranded conformation polymorphism in 50 infants infected congenitally and 25 asymptomatic infants.

Results gN1 (p = 0.010) and UL144-B (p = 0.034) genotypes were associated, by logistic regression, with reduced risk of developing symptomatic congenital CMV infection. gN1 (p = 0.020) and gN3 (p = 0.022) genotypes were associated with reduced risk of severe symptomatic disease. Conversely, gB1 (p = 0.018) was the most virulent genotype and was associated with severe symptoms.

Conclusion An association among gB1, gN1, gN3, and UL144-B genotypes of CMV and severity of congenital CMV disease might exist. gB, gN, and UL144 genotypes could be important virological markers of infant infection.

Keywords

CMV genotypes - symptomatic CMV disease - virulence - congenital infection


Publication History

Received: 15 October 2020

Accepted: 08 March 2021

Article published online:
15 June 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

Georg Thieme Verlag KG
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