CC BY-NC-ND 4.0 · J Lab Physicians 2021; 13(01): 006-013
DOI: 10.1055/s-0041-1726575
Original Article

The Effects of a Single Freeze-Thaw Cycle on Concentrations of Nutritional, Noncommunicable Disease, and Inflammatory Biomarkers in Serum Samples

Ransi Ann Abraham
1  Department of Cardiac Biochemistry, All India Institute of Medical Sciences, Delhi, India
,
Garima Rana
1  Department of Cardiac Biochemistry, All India Institute of Medical Sciences, Delhi, India
,
Praween K. Agrawal
2  UNICEF, Delhi, India
,
Robert Johnston
2  UNICEF, Delhi, India
,
Avina Sarna
3  Population Council, Delhi, India
,
Sowmya Ramesh
3  Population Council, Delhi, India
,
Rajib Acharya
3  Population Council, Delhi, India
,
Nizamuddin Khan
3  Population Council, Delhi, India
,
Akash Porwal
3  Population Council, Delhi, India
,
Sucheta Banerjee Kurundkar
4  Clinical Development Services Agency, Translational Health Science & Technology Institute, Faridabad, Haryana
,
Arvind Pandey
5  National Institute of Medical Statistics, Indian Council of Medical Research, Delhi, India
,
Raghu Pullakhandam
6  National Institute of Nutrition, Hyderabad, Telangana, India
,
Krishnapillai Madhavan Nair
6  National Institute of Nutrition, Hyderabad, Telangana, India
,
Geeta Trilok Kumar
7  Institute of Home economics, Delhi, India
,
HPS Sachdev
8  Paediatrics and Clinical Epidemiology, B-16 Qutab Institutional Area, New Delhi, India
,
Umesh Kapil
9  Department of Human Nutrition, All India Institute of Medical Sciences, Delhi, India
,
Sila Deb
10  Ministry of Health and Family Welfare, Delhi, India
,
Arjan de Wagt
2  UNICEF, Delhi, India
,
Ajay Khera
10  Ministry of Health and Family Welfare, Delhi, India
,
Lakshmy Ramakrishnan
1  Department of Cardiac Biochemistry, All India Institute of Medical Sciences, Delhi, India
› Author Affiliations

Abstract

Background The stability of biological samples is vital for reliable measurements of biomarkers in large-scale survey settings, which may be affected by freeze-thaw procedures. We examined the effect of a single freeze-thaw cycle on 13 nutritional, noncommunicable diseases (NCD), and inflammatory bioanalytes in serum samples.

Method Blood samples were collected from 70 subjects centrifuged after 30 minutes and aliquoted immediately. After a baseline analysis of the analytes, the samples were stored at − 70°C for 1 month and reanalyzed for all the parameters. Mean percentage differences between baseline (fresh blood) and freeze-thaw concentrations were calculated using paired sample t-tests and evaluated according to total allowable error (TEa) limits (desirable bias).

Results Freeze-thaw concentrations differed significantly (p < 0.05) from baseline concentrations for soluble transferrin receptor (sTfR) (− 5.49%), vitamin D (− 12.51%), vitamin B12 (− 3.74%), plasma glucose (1.93%), C-reactive protein (CRP) (3.45%), high-density lipoprotein (HDL) (7.98%), and cholesterol (9.76%), but they were within respective TEa limits. Low-density lipoprotein (LDL) (− 0.67%), creatinine (0.94%), albumin (0.87%), total protein (1.00%), ferritin (− 0.58%), and triglycerides (TAG) (2.82%) concentrations remained stable following the freeze-thaw cycle. In conclusion, single freeze-thaw cycle of the biomarkers in serum/plasma samples after storage at − 70°C for 1 month had minimal effect on stability of the studied analytes, and the changes in concentration were within acceptable limit for all analytes.



Publication History

Publication Date:
24 May 2021 (online)

© 2021. The Indian Association of Laboratory Physicians. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India