CC BY 4.0 · Glob Med Genet 2021; 08(02): 072-077
DOI: 10.1055/s-0041-1726335
Original Article

Granulocytes Acquire Antiapoptosis Activity and Promote Tumor Growth during Tumor Progress

Han Li*
1  Med-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai, People's Republic of China
,
Wenyan Shen*
2  Department of Clinical Laboratory, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, People's Republic of China
,
Yanjie Xu
1  Med-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai, People's Republic of China
,
Zien Wang
1  Med-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai, People's Republic of China
,
Linghao Wang
1  Med-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai, People's Republic of China
,
Zequn Ding
1  Med-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai, People's Republic of China
,
Zhiyuan Xie
1  Med-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai, People's Republic of China
,
Yan Zhang
1  Med-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai, People's Republic of China
› Author Affiliations
Funding None.

Abstract

Granulocytes play important roles in cancer, and their apoptotic status is often changed by the influence of tumor environment. However, the changes and the function on granulocyte apoptosis in cancer are unclear. In this study, we used tumor-bearing mouse model and tumor patients to analyzed the apoptosis of granulocytes in different tissues by flow analysis and TUNEL fluorescence staining, and found that the percentage of apoptosis cells in granulocytes was significantly decreased in late-stage tumor-bearing mouse and patients. The in vitro co-culture experiment showed that these antiapoptotic granulocytes could significantly inhibit T cell proliferation, and RNA-seq proved that there was obvious difference on the transcriptome between these cells and control cells, particularly immune-related genes. What is important, adoptive transfer of these antiapoptotic granulocytes promoted tumor progress in mouse model. Conclusively, we found that granulocytes in late-stage tumor could delay the process of apoptosis, inhibit T cell proliferation, and acquire pro-tumor activity, which provides a new therapeutic target for tumor immunity.

* These authors contributed equally to this work.


Supplementary Material



Publication History

Publication Date:
16 March 2021 (online)

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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