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CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2022; 57(02): 314-320
DOI: 10.1055/s-0041-1724076
Artigo Original
Joelho

BMP-4, TGF-β e Smad3 como moduladores da viabilidade das células do líquido sinovial[*]

Article in several languages: português | English
Eduardo Branco de Sousa 
1   Divisão de Ensino e Pesquisa, Instituto Nacional de Ortopedia e Traumatologia Jamil Haddad, Rio de Janeiro, RJ, Brasil
,
Vivaldo Moura Neto 
2   Laboratório de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Rio de Janeiro, RJ, Brasil
,
Diego Pinheiro Aguiar 
3   Laboratório de Biomodelos e Protótipos, Universidade Estadual da Zona Oeste, Rio de Janeiro, RJ, Brasil
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Resumo

Objetivo Nosso objetivo foi avaliar a modulação das células do líquido sinovial (SFCs, na sigla em inglês) de pacientes com e sem osteoartrite (OA) por proteína morfogenética óssea 4 (BMP-4), Smad3 e transformador do fator de crescimento β (TGF-β).

Métodos O do líquido sinovial foi coletado de pacientes submetidos a artroscopia ou artroplastia do joelho, e centrifugados para isolar as células do liquido sinovial. As células foram cultivadas por 21 dias e caracterizadas como células-tronco mesenquimais (MSCs, na sigla em inglês) de acordo com os critérios da International Society of Cell Therapy. Em seguida, realizamos um ensaio de brometo de 3-4,5-dimetil-tiazol-2-il-2,5-difeniltetrazólio (MTT) depois de expor células com e sem OA para TGF-β, inibidores de via Smad3 e BMP-4 e para diferentes concentrações de BMP-4.

Resultados A exposição aos inibidores TGF-β, Smad3 e BMP-4 modifica a atividade mitocondrial das SFCs. A atividade das SFCs é modificada por influências sobre o aumento das concentrações de BMP-4, mas não há diferença na atividade celular entre pacientes com e sem OA.

Conclusão TGF-β, Smad3 e BMP-4 modulam a atividade das SFCs de pacientes com e sem OA do joelho.

Palavras-chave

células-tronco mesenquimais - osteoartrite - células do líquido sinovial - caminho TGF-β

Contribuições autorais

de Sousa EB Conceito e projeto, fornecimento de material de estudo ou pacientes, coleta e montagem de dados, análise e interpretação de dados, redação de manuscritos, aprovação final do manuscrito.


Moura Neto V.: Análise e interpretação de dados, aprovação final do manuscrito.


Aguiar D. P.: Conceito e projeto, coleta e montagem de dados, análise e interpretação de dados, redação de manuscritos, aprovação final do manuscrito.


Todos os autores leram e aprovaram o manuscrito final.


* Trabalho desenvolvido na Divisão de Ensino e Pesquisa, Instituto Nacional de Ortopedia e Traumatologia Jamil Haddad, Rio de Janeiro, RJ, Brasil.




Publication History

Received: 31 May 2020

Accepted: 16 September 2020

Article published online:
31 March 2021

© 2021. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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