BMP-4, TGF-β e Smad3 como moduladores da viabilidade das células do líquido sinovial^[*]

Resumo

Objetivo Nosso objetivo foi avaliar a modulação das células do líquido sinovial (SFCs, na sigla em inglês) de pacientes com e sem osteoartrite (OA) por proteína morfogenética óssea 4 (BMP-4), Smad3 e transformador do fator de crescimento β (TGF-β).

Métodos O do líquido sinovial foi coletado de pacientes submetidos a artroscopia ou artroplastia do joelho, e centrifugados para isolar as células do liquido sinovial. As células foram cultivadas por 21 dias e caracterizadas como células-tronco mesenquimais (MSCs, na sigla em inglês) de acordo com os critérios da International Society of Cell Therapy. Em seguida, realizamos um ensaio de brometo de 3-4,5-dimetil-tiazol-2-il-2,5-difeniltetrazólio (MTT) depois de expor células com e sem OA para TGF-β, inibidores de via Smad3 e BMP-4 e para diferentes concentrações de BMP-4.

Resultados A exposição aos inibidores TGF-β, Smad3 e BMP-4 modifica a atividade mitocondrial das SFCs. A atividade das SFCs é modificada por influências sobre o aumento das concentrações de BMP-4, mas não há diferença na atividade celular entre pacientes com e sem OA.

Conclusão TGF-β, Smad3 e BMP-4 modulam a atividade das SFCs de pacientes com e sem OA do joelho.

Palavras-chave

células-tronco mesenquimais - osteoartrite - células do líquido sinovial - caminho TGF-β

Contribuições autorais

de Sousa EB Conceito e projeto, fornecimento de material de estudo ou pacientes, coleta e montagem de dados, análise e interpretação de dados, redação de manuscritos, aprovação final do manuscrito.

Moura Neto V.: Análise e interpretação de dados, aprovação final do manuscrito.

Aguiar D. P.: Conceito e projeto, coleta e montagem de dados, análise e interpretação de dados, redação de manuscritos, aprovação final do manuscrito.

Todos os autores leram e aprovaram o manuscrito final.

^* Trabalho desenvolvido na Divisão de Ensino e Pesquisa, Instituto Nacional de Ortopedia e Traumatologia Jamil Haddad, Rio de Janeiro, RJ, Brasil.

Publication History

Received: 31 May 2020

Accepted: 16 September 2020

Article published online:
31 March 2021

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