Individuals Age Determination from Human Dental Pulp Through DNA Analysis by PCR

M.L. Avinash Tejasvi; Anulekha Avinash CK; E. Rajendra Reddy; Pavan Kulkarni; Harsha Bhayya; Manohar S. Kugaji

doi:10.1055/s-0041-1723084

Global Medical Genetics, Inhaltsverzeichnis

CC BY 4.0 · Glob Med Genet 2021; 08(02): 057-061
DOI: 10.1055/s-0041-1723084

Original Article

Individuals Age Determination from Human Dental Pulp Through DNA Analysis by PCR

M.L. Avinash Tejasvi

¹Department of Oral Medicine and Radiology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India

,

Anulekha Avinash CK

²Department of Prosthodontics, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India

,

E. Rajendra Reddy

³Department of Pedodontics, Kamineni Institute Dental Sciences, Narketpally, Telangana, India

,

Pavan Kulkarni

⁴Department of Oral Pathology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India

,

Harsha Bhayya

¹Department of Oral Medicine and Radiology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India

,

Manohar S. Kugaji

⁵Department of Genetics, Central Research Laboratory, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India

› Institutsangaben

Abstract

Objectives Age estimation in forensic odontology is having a great importance in recent times because of the request by court or other government authorities so that immigrants whose real age is unknown should not suffer unfair disadvantages because of their supposed age, and so that all legal procedures to which an individual's age is relevant can be properly followed.

Purpose The present study was planned to be conducted on pulp tissue and dental hard tissues derived from individuals for DNA isolation and age determination .

Materials and Methods The present study was an experimental single-blinded study consisting of 30 extracted teeth categorized into three groups as follows: Group A: 10 to 20 years, Group B: 21 to 30 years, Group C: 31 to 40 years. DNA was isolated from the pulp of each tooth and quantitative polymerase chain reaction (qPCR) for calculating telomere length was performed.

Results With increase in age, the length of telomere gets shortened which will be helpful in analyzing the age of the person when morphological and biological remnants are not available except the tooth.

Conclusion The present study found that estimation of human age based on the relative TL measured by the real-time quantitative PCR may be a useful method for age prediction, especially when there is no morphologic information in the biological sample. This is the first study to accesses the age of a person by telomere length using dental pulp.

Keywords

age - PCR - pulp - dentin

Volltext

Referenzen

References
1 Sun H-W, Gao C-R. The application of telomere DNA in age estimation of forensic medicine. Fa Yi Xue Za Zhi 2005; 21 (02) 155-158
2 Vanpelt E, Belkum VAV, Hays JP. Principles and Technical Aspects of PCR Amplification. Netherlands: Springer; 2008: 34
3 Khorate MM, Dhupar A, Ahmed J, Dinkar AD. Gender determination from pulpal tissue. J Forensic Dent Sci 2014; 6 (02) 107-112
4 O'Callaghan N, Dhillon V, Thomas P, Fenech M. A quantitative real-time PCR method for absolute telomere length. Biotechniques 2008; 44 (06) 807-809
5 Shay JW, Wright WE. Hallmarks of telomeres in ageing research. J Pathol 2007; 211 (02) 114-123
6 Ata-Ali J, Ata-Ali F. Forensic dentistry in human identification: a review of the literature. J Clin Exp Dent 2014; 6 (02) e162-e167
7 Naik PR, Acath DD, Sharma GH, Navalkar AR. Viability of human dental pulp in determination of sex of an individual by identifying SRY gene through DNA analysis: a single blind pilot study. JIAOMR 2012; 24: 133-136
8 Khan R, Tejasvi MLA, Paramkusam G. Comparison of gender determination from dental pulp and dentin after exposure to various environmental conditions: a polymerase chain reaction-based SRY gene study. Contemp Clin Dent 2019; 10 (02) 256-262
9 Battepati PM, Shodan M. Gender determination using primary teeth: a polymerase chain reaction (PCR) study. J Dent Oral Hyg 2013; 5: 77-82
10 Shammas MA. Telomeres, lifestyle, cancer, and aging. Curr Opin Clin Nutr Metab Care 2011; 14 (01) 28-34