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CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(03): 546-550
DOI: 10.1055/s-0041-1723067
Original Article

Bactericidal Effect of a Novel Alkaline EDTA Root Canal Cleaning Solution

Takenori Sato
1   Department of Oral Science, Division of Oral Biochemistry, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
,
Ryuji Fujimaki
2   Department of Oral Interdisciplinary Medicine, Division of Pulp Biology, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
,
Jiro Suzuki
2   Department of Oral Interdisciplinary Medicine, Division of Pulp Biology, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
,
Nobushiro Hamada
3   Department of Oral Science, Division of Microbiology, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
,
Nobuyuki Tani-Ishii
2   Department of Oral Interdisciplinary Medicine, Division of Pulp Biology, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
,
Keisuke Handa
1   Department of Oral Science, Division of Oral Biochemistry, Graduate School of Dentistry, Kanagawa Dental University, Kanagawa, Japan
› Author Affiliations Funding This work was supported by JSPS KAKENHI under grant numbers JP16K11568, and JP18K09592.
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Abstract

Objectives In this study, we aimed to evaluate the bactericidal effect and cytotoxicity of an ethylenediaminetetra-acetic acid (EDTA)-based root canal irrigant solution capable of efficiently removing both the organic matter and the smear layer. We prepared a strong alkaline EDTA (AE) solution with an acid buffer capacity similar to that of sodium hypochlorite.

Materials and Methods AE was used at concentrations of 1%, 2%, and 3%. The bactericidal effect of AE on Enterococcus faecalis was evaluated by determining the colony number and biofilm removal rate. Biofilms were visualized using a Live/Dead BacLight bacterial viability kit. Viability of AE-treated cells were determined using a CCK-8 cell counting assay.

Statistical Analysis One-way analysis of variance followed by a Dunnett’s multiple comparison test were used for comparisons among groups.

Results Significant reduction in cell viability and biofilm formation were observed in case of 3% and 2% AE. AE exerted bactericidal effects in a concentration-dependent manner. Damage of normal human fibroblasts was not observed at any of the AE concentrations.

Conclusions Our results suggest that the AE solution could be used as an effective canal irrigant for the removal of bacterial biofilm.

Keywords

ethylenediaminetetraacetic acid - sodium hypochlorite - biofilm - root canal irrigant


Publication History

Article published online:
10 March 2021

© 2021. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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  • References

  • 1 Siqueira Jr JF, Araújo MC, Garcia PF, Fraga RC, Dantas CJ. Histological evaluation of the effectiveness of five instrumentation techniques for cleaning the apical third of root canals. J Endod 1997; 23 (08) 499-502
    CrossrefPubMedGoogle Scholar
  • 2 Hülsmann M, Peters OA, Dummer PMH. Mechanical preparation of root canals: shaping goals, techniques and means. Endod Topics 2005; 10 (01) 30-76
    CrossrefGoogle Scholar
  • 3 Peters OA, Schönenberger K, Laib A. Effects of four Ni-Ti preparation techniques on root canal geometry assessed by micro computed tomography. Int Endod J 2001; 34 (03) 221-230
    CrossrefPubMedGoogle Scholar
  • 4 Peters OA, Peters CI, Schönenberger K, Barbakow F. ProTaper rotary root canal preparation: effects of canal anatomy on final shape analysed by micro CT. Int Endod J 2003; 36 (02) 86-92
    CrossrefPubMedGoogle Scholar
  • 5 von Arx T. Frequency and type of canal isthmuses in first molars detected by endoscopic inspection during periradicular surgery. Int Endod J 2005; 38 (03) 160-168
    CrossrefPubMedGoogle Scholar
  • 6 Haapasalo M, Shen Y, Qian W, Gao Y. Irrigation in endodontics. Dent Clin North Am 2010; 54 (02) 291-312
    CrossrefPubMedGoogle Scholar
  • 7 Dioguardi M, Gioia GD, Illuzzi G, Laneve E, Cocco A, Troiano G. Endodontic irrigants: different methods to improve efficacy and related problems. Eur J Dent 2018; 12 (03) 459-466
    Article in Thieme ConnectPubMedGoogle Scholar
  • 8 Hülsmann M, Hahn W. Complications during root canal irrigation–literature review and case reports. Int Endod J 2000; 33 (03) 186-193
    CrossrefPubMedGoogle Scholar
  • 9 Guivarc’h M, Ordioni U, Ahmed HM, Cohen S, Catherine JH, Bukiet F. Sodium hypochlorite accident: a systematic review. J Endod 2017; 43 (01) 16-24
    CrossrefPubMedGoogle Scholar
  • 10 Dioguardi M, Di Gioia G, Illuzzi G. et al. Inspection of the microbiota in endodontic lesions. Dent J (Basel) 2019; 7 (02) E47
    CrossrefPubMedGoogle Scholar
  • 11 Ran S, Gu S, Wang J, Zhu C, Liang J. Dentin tubule invasion by Enterococcus faecalis under stress conditions ex vivo. Eur J Oral Sci 2015; 123 (05) 362-368
    CrossrefPubMedGoogle Scholar
  • 12 Barbosa-Ribeiro M, De-Jesus-Soares A, Zaia AA, Ferraz CC, Almeida JF, Gomes BP. Antimicrobial susceptibility and characterization of virulence genes of enterococcus faecalis isolates from teeth with failure of the endodontic treatment. J Endod 2016; 42 (07) 1022-1028
    CrossrefPubMedGoogle Scholar
  • 13 Beomidehagh M, Rezaee MA, Ganbarov K. et al. Effect of acidic and alkali shocks on expression of efaA gene in Enterococcus faecalis, isolated from root canal infection. Cell Mol Biol 2018; 64 (13) 1-5
    CrossrefPubMedGoogle Scholar
  • 14 Tian Y, Zhang X, Zhang K. et al. Effect of Enterococcus faecalis lipoteichoic acid on apoptosis in human osteoblast-like cells. J Endod 2013; 39 (05) 632-637
    CrossrefPubMedGoogle Scholar
  • 15 Wang L, Jin H, Ao X. et al. JAK2-STAT3 signaling pathway is involved in rat periapical lesions induced by Enterococcus faecalis. Oral Dis 2019; 25 (07) 1769-1779
    CrossrefPubMedGoogle Scholar
  • 16 Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006; 32 (02) 93-98
    CrossrefPubMedGoogle Scholar
  • 17 Solana C, Ruiz-Linares M, Baca P, Valderrama MJ, Arias-Moliz MT, Ferrer-Luque CM. Antibiofilm activity of sodium hypochlorite and alkaline tetrasodium EDTA solutions. J Endod 2017; 43 (12) 2093-2096
    CrossrefPubMedGoogle Scholar
  • 18 Vajrabhaya LO, Sangalungkarn V, Srisatjaluk R, Korsuwannawong S, Phruksaniyom C. Hypochlorite solution for root canal irrigation that lacks a chlorinated odor. Eur J Dent 2017; 11 (02) 221-225
    Article in Thieme ConnectPubMedGoogle Scholar
  • 19 Segu A, Bijukumar D, Trinh T. et al. Total eradication of bacterial infection in root canal treatment: an electrochemical approach. ACS Biomater Sci Eng 2018; 4 (07) 2623-2632
    CrossrefPubMedGoogle Scholar
  • 20 Slaughter RJ, Watts M, Vale JA, Grieve JR, Schep LJ. The clinical toxicology of sodium hypochlorite. Clin Toxicol (Phila) 2019; 57 (05) 303-311
    CrossrefPubMedGoogle Scholar
  • 21 De Moor RJ, Blanken J, Meire M, Verdaasdonk R. Laser induced explosive vapor and cavitation resulting in effective irrigation of the root canal. Part 2: evaluation of the efficacy. Lasers Surg Med 2009; 41 (07) 520-523
    CrossrefPubMedGoogle Scholar
  • 22 Meire MA, Poelman D, De Moor RJ. Optical properties of root canal irrigants in the 300-3,000-nm wavelength region. Lasers Med Sci 2014; 29 (05) 1557-1562
    CrossrefPubMedGoogle Scholar
  • 23 Giardino L, Del Fabbro M, Cesario F, Fernandes FS, Andrade FB. Antimicrobial effectiveness of combinations of oxidant and chelating agents in infected dentine: an ex vivo confocal laser scanning microscopy study. Int Endod J 2018; 51 (04) 448-456
    CrossrefPubMedGoogle Scholar
  • 24 Gu LS, Kim JR, Ling J, Choi KK, Pashley DH, Tay FR. Review of contemporary irrigant agitation techniques and devices. J Endod 2009; 35 (06) 791-804
    CrossrefPubMedGoogle Scholar
  • 25 van der Sluis LW, Versluis M, Wu MK, Wesselink PR. Passive ultrasonic irrigation of the root canal: a review of the literature. Int Endod J 2007; 40 (06) 415-426
    CrossrefPubMedGoogle Scholar
  • 26 Virdee SS, Seymour DW, Farnell D, Bhamra G, Bhakta S. Efficacy of irrigant activation techniques in removing intracanal smear layer and debris from mature permanent teeth: a systematic review and meta-analysis. Int Endod J 2018; 51 (06) 605-621
    CrossrefPubMedGoogle Scholar