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CC BY 4.0 · Glob Med Genet 2020; 07(04): 113-120
DOI: 10.1055/s-0041-1722884
Original Article

The Connotation of Variances in the Risk Predictors, Medications, Homocysteine, and Homocysteine Pathway Gene Polymorphisms with CVA/Stroke

Rizwan Masud
1   Department of Physiology, CMH Kharian Medical College, Kharian, Pakistan
,
Aleem Ul Haq Khan
2   Department of Biochemistry, CMH Kharian Medical College, Kharian, Pakistan
,
Aiman Farogh Anjum
1   Department of Physiology, CMH Kharian Medical College, Kharian, Pakistan
,
Ghazala Jawwad
3   Department of Physiology, Rawal Institute of Health Sciences, Islamabad, Pakistan
,
Zahid Azeem
4   Department of Biochemistry, AJ&K Medical College, Muzaffarabad, AJ&K, Pakistan
,
Haider Zaigham Baqai
5   Department of Medicine, Nafees Medical College, Islamabad, Pakistan
,
Shoaib Naiyar Hashmi
6   Department of Pathology, CMH Kharian Medical College, Kharian, Pakistan
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Abstract

Cerebrovascular accidents (CVAs) are vascular multifactorial, multigenic ailments with intricate genetic, environmental risk influences. The present study aimed to establish affiliation of CVAs/stroke with blood parameters, differences in prescribed drugs consumption, and with differences in homocysteine pathway genes polymorphisms. The participants in study included controls n = 251, transient ischemic attack (TIA) patients n = 16, and stroke cases n = 122, respectively, (total participants, n = 389). The analyzed single nucleotide polymorphisms (SNPs) included C677T(rs1801133), A1298C(rs1801131) of methylene tetrahydrofolate reductase (MTHFR), A2756G(rs1805087) of methyl tetrahydrofolate homocysteine methyltransferase/methionine synthase (MS), and the A192G(rs662) of paraoxonase 1(PON1) genes, all validated by tetra-primer allele refractory mutation system polymerase chain reaction (T-ARMS-PCR). The insertion deletion (I/D; rs4646994) polymorphism in angiotensin converting enzyme (ACE) gene was analyzed using routine PCR. All studied traits were scrutinized through analysis of variance (ANOVA), and later through regression analysis. Through ANOVA and multiple comparison, there was association of CVA with serum homocysteine, cholesterol, and with diastolic blood pressure readings. When data was subjected to regression, serum homocysteine and diastolic blood pressure (significant through ANOVA), as well as two additional traits, high-density lipoproteins (HDL), and rs1801133 MTHFR SNP sustained statistical significance and noteworthy odds in relation to CVA and stroke. The ailments affecting cerebral vasculature are mutifactorial, whereby genes, proteins, and environmental cues all exert cumulative effects enhancing CVA risk. The current study emphasizes that SNPs and variation in circulating biomarkers can be used for screening purposes and for reviewing their effects in stroke/CVA-linked risk progression.

Keywords

cerebrovascular accidents - single nucleotide polymorphisms - stroke - transient ischemic attack - homocysteine


Publication History

Article published online:
09 February 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Georg Thieme Verlag KG
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