Geburtshilfe Frauenheilkd 2016; 76(06): 709-717
DOI: 10.1055/s-0041-111697
Original Article
GebFra Science
Georg Thieme Verlag KG Stuttgart · New York

Osteopontin and Integrin αvβ3 Expression during the Implantation Window in IVF Patients with Elevated Serum Progesterone and Oestradiol Level

Expression von Osteopontin und Integrin αvβ3 während des Implantationsfensters in IVF-Patientinnen mit erhöhten Progesteron- und Östradiolwerten
Z. He*
1   Department of Reproduction and Genetics, Reproductive Medical Centre, The Affiliated Hospital of Kunming University of Science and Technology, The First Peopleʼs Hospital of Yunnan Province, Kunming, Peopleʼs Republic of China
,
Y. Ma*
1   Department of Reproduction and Genetics, Reproductive Medical Centre, The Affiliated Hospital of Kunming University of Science and Technology, The First Peopleʼs Hospital of Yunnan Province, Kunming, Peopleʼs Republic of China
,
L. Li
1   Department of Reproduction and Genetics, Reproductive Medical Centre, The Affiliated Hospital of Kunming University of Science and Technology, The First Peopleʼs Hospital of Yunnan Province, Kunming, Peopleʼs Republic of China
,
J. Liu
3   Institute of Molecular and Clinical Medicine, Kunming Medical University, Chengong New District, Kunming, Peopleʼs Republic of China
,
H. Yang
2   Department of Pathology, The First Peopleʼs Hospital of Yunnan Province, Kunming, Peopleʼs Republic of China
,
C. Chen
4   Department of Obstetrics and Gynecology, Reproductive Medical Centre, Peking University Third Hospital, Haidian District, Beijing, Peopleʼs Republic of China
,
N. Lin
1   Department of Reproduction and Genetics, Reproductive Medical Centre, The Affiliated Hospital of Kunming University of Science and Technology, The First Peopleʼs Hospital of Yunnan Province, Kunming, Peopleʼs Republic of China
,
Y. Bai
1   Department of Reproduction and Genetics, Reproductive Medical Centre, The Affiliated Hospital of Kunming University of Science and Technology, The First Peopleʼs Hospital of Yunnan Province, Kunming, Peopleʼs Republic of China
,
R. Ma
1   Department of Reproduction and Genetics, Reproductive Medical Centre, The Affiliated Hospital of Kunming University of Science and Technology, The First Peopleʼs Hospital of Yunnan Province, Kunming, Peopleʼs Republic of China
,
R. Li
4   Department of Obstetrics and Gynecology, Reproductive Medical Centre, Peking University Third Hospital, Haidian District, Beijing, Peopleʼs Republic of China
,
Z. Wu
1   Department of Reproduction and Genetics, Reproductive Medical Centre, The Affiliated Hospital of Kunming University of Science and Technology, The First Peopleʼs Hospital of Yunnan Province, Kunming, Peopleʼs Republic of China
,
J. Qiao
4   Department of Obstetrics and Gynecology, Reproductive Medical Centre, Peking University Third Hospital, Haidian District, Beijing, Peopleʼs Republic of China
› Author Affiliations
Further Information

Publication History

received 23 September 2015
revised 20 December 2015

accepted 20 December 2015

Publication Date:
27 June 2016 (online)

Abstract

Background: To explore whether endometrial receptivity is determined by osteopontin (OPN) and integrin αvβ3 expression in women with elevated serum progesterone (P) and/or oestradiol (E2) who are undergoing in vitro fertilisation (IVF). Methods: According to serum hormone levels on the day of HCG administration, 33 infertile women were divided into 3 groups: the high E2, high P, and high E2 and P groups. The control group included 11 fertile, healthy women. Endometrial biopsy was performed on ovulation day + 7 to + 8 for all study participants, and the mRNA and protein expression levels of OPN and integrin αvβ3 were analyzed. Result: No statistically significant differences regarding OPN and integrin αvβ3 expression were found between infertile patients in the high P, high E2, high E2 and P and control groups. There was no significant correlation between OPN and integrin αvβ3 staining intensity during the implantation window biopsy in any of the groups studied. Conclusion: Endometrial OPN and integrant αvβ3 expression/co-expression is not impaired during the window of implantation in patients with high P, high E2, or high E2 and P levels. The clinical value of assessing endometrial receptivity with OPN and integrin αvβ3 seems to be uncertain.

Zusammenfassung

Hintergrund: Ziel der Studie war es zu untersuchen, ob die Rezeptivität des Endometriums von der Osteopontin- (OPN-) und Integrin αvβ3-Expression bestimmt wird bei Frauen mit erhöhten Progesteron-(P-) und/oder Östradiol-(E2-)Konzentrationen, die sich einer In-vitro-Fertilisation (IVF) unterziehen. Methoden: Basierend auf der Messung des Hormonspiegels am Tage der hCG-Gabe wurden 33 unfruchtbare Frauen in 3 Gruppen wie folgt eingeteilt: hoher E2-, hoher P- und hoher E2- und P-Spiegel. Die Kontrollgruppe bestand aus 11 gesunden fruchtbaren Frauen. Eine Biopsie des Endometriums wurde am 7. oder 8. Tag nach der Ovulation bei allen Studienteilnehmende durchgeführt, und die mRNA und Protein-Expressionsspiegeln von OPN und Integrin αvβ3 wurden ausgewertet. Ergebnisse: Bezüglich der Exprimierung von OPN und Integrin αvβ3 fanden sich keine statistisch signifikanten Unterschiede zwischen den unfruchtbaren Patientinnen in den Gruppen mit hohen P- oder E2-Spiegeln, der Gruppe mit hohen E2- und P-Spiegeln und der Kontrollgruppe. In den entnommenen Biopsaten konnte bei keiner der untersuchten Gruppen eine signifikante Korrelation zwischen der Färbungsintensität für OPN und Integrin αvβ3 und dem Implantationsfenster festgestellt werden. Schlussfolgerungen: Die Expression und Koexpression von OPN und Integrin αvβ3 im Endometrium sind während des Implantationsfensters nicht beeinträchtigt bei Patientinnen mit hohen P- oder E2-Spiegeln bzw. hohen E2- und P-Spiegeln. Der klinische Nutzen einer auf die Messung der Expression von OPN- und Integrin αvβ3 basierenden Bewertung der Rezeptivität des Endometriums scheint fraglich zu sein.

* Co-author: Zhenlin He and Yanping Ma are joint first authors of this article.