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CC BY-NC-ND 4.0 · Journal of Coloproctology 2021; 41(01): 063-069
DOI: 10.1055/s-0040-1722562
Original Article

Increased Expression of Type-I Collagen in Malignant Colorectal Lesion and Positive Correlations with Clinical Factors[*]

Expressão aumentada do colágeno tipo I na lesão colorretal maligna e correlações positivas com fatores clínicos
Monique Pinheiro Santos
1   Faculdade de Medicina, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brazil
,
Izabela Silva Sinara Alves
1   Faculdade de Medicina, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brazil
,
Willian Grassi Bautz
2   Departament of Morphology, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brazil
,
Flavya da Silva Souza Ribeiro
3   Laboratório PAT, Vitória, ES, Brazil
,
Luciano Pinto Nogueira da Gama
4   Departament of Coloproctology, Vitória Apart Hospital, Serra, ES, Brazil
,
Raquel Spinassé Dettogni
2   Departament of Morphology, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brazil
,
Letícia Nogueira da Gama-de-Souza 
2   Departament of Morphology, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brazil
› Author Affiliations Funding This work was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) under process number: 479694 2013-3, and by Fundação de Amparo à Pesquisa e Inovação do Espírito Santo (FAPES) under process number: 67659870 006/2014.
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Abstract

Objective Type-I collagen (Col-I) is one of the main macromolecules of the extracellular matrix, and it is involved in the desmoplastic stromal reaction, an indicator of worse prognosis in cases of colorectal cancer (CRC). The purpose of the present study was to investigate Col-I expression in cases of CRC and adenoma and to correlate with the clinical data and the data regarding the lifestyle of the patients.

Methods A retrospective study including 22 patients with adenoma and 15 with CRC treated at a coloproctology service. The clinical and lifestyle data were obtained through medical records, and Col-I expression was investigated by immunohistochemistry.

Results Women represented most cases of adenoma (63.64%), whereas CRC was found mainly in men (73.33%) (p = 0.0448). Immunoexpression of Col-I showed a basement membrane thickening in areas of lining of epithelium and around the glands in both lesions. The cases of CRC had a quite evident fibrosis process in the stroma. The quantitative analysis demonstrated a higher protein expression in CRCs compared to adenomas (p = 0.0109), as well as in female patients (p = 0.0214), patients aged ≥ 50 years (p = 0.0400), and in those with a positive family history of colorectal disease (p = 0.0292). These results suggested a remodeling of the microenvironment of the tumor in CRC carcinogenesis. Importantly, the clinicopathologic positive correlations showed a plausible link between the patient's profile and the immunohistochemical findings, which indicate a possible form of patient stratification.

Conclusion The immunohistochemical analysis encourages the performance of more comprehensive studies to ascertain if our results could be a tool for the diagnosis and monitoring of the patients.

Resumo

Objetivo O colágeno tipo I (Col-I) é uma das principais macromoléculas da matriz extracelular, e está envolvido na reação desmoplástica estromal, um indicador de pior prognóstico em casos de câncer colorretal (CCR). O objetivo foi investigar a expressão do Col-I em casos de CCR e adenoma, e correlacioná-la com dados clínicos e de estilo de vida dos pacientes.

Metodologia Foi realizado um estudo retrospectivo com 22 pacientes com adenoma e 15 com CCR tratados em um serviço de coloproctologia. Os dados dos pacientes foram obtidos dos prontuários médicos, e a expressão do Col-I foi investigada por imunohistoquímica.

Resultados As mulheres representaram a maioria dos casos de adenomas (63,64%), enquanto o CCR (73,33%) (p = 0,0448) foi mais comum entre os homens. A imunoexpressão de Col-I mostrou espessamento da membrana basal em áreas de revestimento do epitélio e em volta de glândulas em ambas as lesões. O CCR apresentou fibrose no estroma. As análises quantitativas demonstraram maior expressão proteica no CCR (p = 0,0109), assim como em mulheres (p = 0,0214), pacientes com idade ≥ 50 anos (p = 0,0400), e em pacientes com histórico positivo de doença colorretal na família (p = 0,0292). Estes resultados sugerem a remodelação do microambiente tumoral na carcinogênese do CCR. As correlações clínico-patológicas positivas mostram uma ligação plausível entre o perfil do paciente e os achados imunohistoquímcos, o que indica uma possível forma de estratificação dos pacientes.

Conclusão As análises imunohistoquímicas estimulam a execução de estudos mais abrangentes para confirmar se nossos resultados poderão ser uma ferramenta para o diagnóstico e o monitoramento dos pacientes.

Keywords

colorectal cancer - colorectal adenoma - type-I collagen - extracellular matrix

Palavras-chave

câncer colorretal - adenoma colorretal - colágeno tipo I - matriz extracelular

* Worked developed at the Department of Morphology, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, ES, Brazil.




Publication History

Received: 09 August 2020

Accepted: 30 August 2020

Article published online:
19 March 2021

© 2021. Sociedade Brasileira de Coloproctologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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