Thromb Haemost 2021; 121(04): 484-494
DOI: 10.1055/s-0040-1718733
Cellular Haemostasis and Platelets

Impaired Platelet Function in Sept8-Deficient Mice In Vitro

Katharina Neubauer*
1  Division of Pediatric Hematology and Oncology, Department of Pediatrics and Adolescent Medicine, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany
,
Kerstin Jurk*
2  Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
,
Victoria Petermann
2  Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
,
Elena Kumm
2  Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
,
Barbara Zieger
1  Division of Pediatric Hematology and Oncology, Department of Pediatrics and Adolescent Medicine, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany
› Institutsangaben
Funding This work was funded by Deutsche Forschungsgemeinschaft DFG (ZI 486/4-1 and ZI 486/8-1) and by the German Federal Ministry of Education and Research (BMBF 01EO1503). We thank PD Dr. Hauke Werner (Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, Germany) for providing the Sept8 −/− mouse and Eileen Lerner and Anja Kahle for outstanding technical assistance.

Abstract

Septins (Septs) are a widely expressed protein family of 13 mammalian members, recognized as a unique component of the cytoskeleton. In human platelets, we previously described that SEPT4 and SEPT8 are localized surrounding α-granules and move to the platelet surface after activation, indicating a possible role in platelet physiology. In this study, we investigated the impact of Sept8 on platelet function in vitro using Sept8-deficient mouse platelets. Deletion of Sept8 in mouse platelets caused a pronounced defect in activation of the fibrinogen receptor integrin αIIbβ3, α-granule exocytosis, and aggregation, especially in response to the glycoprotein VI agonist convulxin. In contrast, δ-granule and lysosome exocytosis of Sept8-deficient platelets was comparable to wild-type platelets. Sept8-deficient platelet binding to immobilized fibrinogen under static conditions was diminished and spreading delayed. The procoagulant activity of Sept8-deficient platelets was reduced in response to convulxin as determined by lactadherin binding. Also thrombin generation was decreased relative to controls. Thus, Sept8 is required for efficient integrin αIIbβ3 activation, α-granule release, platelet aggregation, and contributes to platelet-dependent thrombin generation. These results revealed Sept8 as a modulator of distinct platelet functions involved in primary and secondary hemostatic processes.

* These authors contributed equally to this work.


Supplementary Material



Publikationsverlauf

Eingereicht: 17. Juli 2020

Angenommen: 11. September 2020

Publikationsdatum:
17. November 2020 (online)

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