The role of follicular Anti-Mullerian hormone in woman undergoing IVF/ICSI with regard to size, oocyte, sociodemographic parameters and other hormones and vitamins

Objective Although the value of serum Anti-Mullerian hormone (AMH) is well known, the influence of AMH on the follicular level has not yet been sufficiently clarified. The aim of this study was to investigate how AMH behaves in terms of follicle size, the presence of an oocyte, sociodemographic parameters and other hormones and vitamins.

Material/Methods We investigated the follicular fluid AMH (fAMH) and serum AMH (sAMH) of 61 female fertility patients undergoing IVF or ICSI therapy (fAMH n= 376, sAMH n=44). On the day of oocyte retrieval, we collected the follicular fluid (FF) of each follicle separately and also a serum sample. The samples were analyzed regarding their AMH level and other hormone concentrations. The follicles were divided into groups (large≥16mm, small< 16mm) and the presence of oocytes.

Results Follicles with a large diameter contained a significantly lower concentration of fAMH (p=0.001). Follicles that contain an oocyte showed a significantly higher fAMH level than those without one (p< 0.001). We also showed that there is a significantly but weak correlation between fAMH and sociodemographic parameters such as the patients age (r=-0.11, p< 0.001) and BMI (r=0.03, p< 0.006). The same applies to the connection between fAMH and other parameters such as Vitamin D (r=-0.13, p< 0.001), LH (r=-0.35, p< 0.001) and progesterone (r=-0.21, p< 0.001) in FF.

Conclusion Our results confirm that larger follicles don´t produce more fAMH than smaller ones. We were able to demonstrate that the presence of an oocyte is significantly associated with the level of fAMH.

Publication History

Article published online:
07 October 2020