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CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(02): 236-241
DOI: 10.1055/s-0040-1716781
Original Article

Biomimetic Mechanism of Action of Fluoridated Toothpaste Containing Proprietary REFIX Technology on the Remineralization and Repair of Demineralized Dental Tissues: An In Vitro Study

Fabiano Vieira Vilhena
1   Trials Pesquisa e Desenvolvimento, Bauru, SP, Brasil
,
Sonia Mariza Luiz de Oliveira
2   Graduate Program in Biotechnology and Health Innovation, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil
,
Marcelo Henrick Maia Matochek
2   Graduate Program in Biotechnology and Health Innovation, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil
,
Pedro Luiz Santos Tomaz
2   Graduate Program in Biotechnology and Health Innovation, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil
,
Thales de Sá Oliveira
2   Graduate Program in Biotechnology and Health Innovation, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil
,
Paulo Henrique Perlatti D’Alpino 
2   Graduate Program in Biotechnology and Health Innovation, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil
3   Graduate Program in Science Teaching and Health, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil
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Abstract

Objectives This in vitro study aimed to characterize the mineral content and surface and cross-sectional morphology of enamel and dentin tissues treated with a 1450 ppm fluoride-containing toothpaste with REFIX technology.

Materials and Methods Bovine enamel blocks (n = 5) were obtained (4 × 4 × 6 mm), demineralized (artificial caries lesion), and treated (pH cycling and brushing with the toothpaste). During the pH cycling, which lasted for 7 days (demineralization and remineralization took 6 and 18 hours, respectively), the enamel was brushed for 5 minutes using an electric toothbrush before being immersed in a remineralizing solution. The dentin blocks were acid-etched for 2 minutes (0.05 M citric acid, pH 1.8) to expose the dentinal tubules (n = 5). Morphological analysis of the dentin was performed immediately and after 7 days of brushing with the dentifrice, and compared with the control group. The specimens were then cross-sectioned. The surface and cross-sectional micromorphology were assessed using scanning electron microscopy (SEM). The elemental analyses (weight%) were determined with an energy-dispersive X-ray spectroscopy (EDS).

Results The toothpaste with REFIX technology remineralized and repaired the surface enamel effectively. The elemental analysis also demonstrated that treating the enamel with the toothpaste formed a silicon-enriched mineral layer on the enamel surface. Elemental analysis of the enamel cross-sections showed that the toothpaste induced a mineral change. The results were also consistent in the dentin, where the dentinal tubules were progressively occluded until there was complete occlusion after 7 days.

Conclusions We prove the biomimetic mechanism of action of fluoridated toothpaste containing proprietary REFIX technology for obtaining silicon-enriched, remineralized and repaired dental tissues.

Keywords

enamel - dentin - tooth remineralization - toothpaste - microscopy - electron - scanning


Publication History

Article published online:
26 November 2020

© 2020. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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