Homeopathy in Experimental Cancer Models: A Systematic Review
Background Complementary and alternative medicine, including homeopathy, is widely used to improve well-being among cancer patients and reduce adverse effects of conventional treatment. In contrast, there are few studies on the use of homeopathic medicines to treat the disease itself. Yet, evidence of possible effectiveness of homeopathic high dilutions in experimental cancer models has been published during the past 20 years.
Aim The aim of the study was to perform a systematic review of fundamental research studies on homeopathic high dilutions in cancer.
Methods Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline, we conducted a literature search in the database PubMed for original publications, from 2000 to 2018 and in English, on in vitro and in vivo experimental cancer models testing homeopathic high dilutions.
Results Twenty-three articles met the inclusion criteria—14 in vitro, eight in vivo, and one in vitro plus in vivo experimental models. Most studies were from India. Research prominently focused on cytotoxic effects involving apoptotic mechanisms. Intrinsic aspects of homeopathy should be considered in experimental designs to emphasize the specificity of such effects.
Conclusion Fundamental research of homeopathy in cancer is still at an early stage and has mainly been performed by a few groups of investigators. The results point to an interference of well-selected homeopathic medicines with cell cycle and apoptotic mechanisms in cancer cells. However, these findings still need independent reproduction.
• Effectiveness of homeopathic products on cancer has been experimentally tested since the early 2000s.
• A systematic review of these studies was performed using PRISMA methods.
• Cell cycle arrest and increase in apoptosis rate were the most reported findings.
• Methodological deficiencies were revealed among the studies.
• Recommendations for further studies are suggested.
Eingereicht: 13. April 2020
Angenommen: 15. Juni 2020
21. Dezember 2020 (online)
© 2020. Faculty of Homeopathy. This article is published by Thieme.
Georg Thieme Verlag KG
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- 1 World Health Organization. Cancer, key facts (2018). Accessed April 5, 2020 at: https://www.who.int/news-room/fact-sheets/detail/cancer
- 2 Rostock M, Naumann J, Guethlin C, Guenther L, Bartsch HH, Walach H. Classical homeopathy in the treatment of cancer patients—a prospective observational study of two independent cohorts. BMC Cancer 2011; 11: 19
- 3 Fulop JA, Grimone A, Victorson D. Restoring balance for people with cancer through integrative oncology. Prim Care 2017; 44: 323-335
- 4 Rossi E, Di Stefano M, Firenzuoli F, Monechi MV, Baccetti S. Add-on complementary medicine in cancer care: evidence in literature and experiences of integration. Medicines (Basel) 2017; 4: 5
- 5 Rossi E, Noberasco C, Picchi M. et al. Complementary and alternative medicine to reduce adverse effects of anti-cancer therapy. J Altern Complement Med 2018; 24: 933-941
- 6 Bosacki C, Vallard A, Gras M. et al. Les médecines alternatives complémentaires en oncologie. Bull Cancer 2019; 106: 479-491
- 7 Kacel EL, Pereira DB, Estores IM. Advancing supportive oncology care via collaboration between psycho-oncology and integrative medicine. Support Care Cancer 2019; 27: 3175-3178
- 8 Newsweek. World's best hospitals 2019. Accessed April 4, 2020 at: https://www.newsweek.com
- 9 Karp JC, Sanchez C, Guilbert P, Mina W, Demonceaux A, Curé H. Treatment with Ruta graveolens 5CH and Rhus toxicodendron 9CH may reduce joint pain and stiffness linked to aromatase inhibitors in women with early breast cancer: results of a pilot observational study. Homeopathy 2016; 105: 299-308
- 10 Sorrentino L, Piraneo S, Riggio E. et al. Is there a role for homeopathy in breast cancer surgery? A first randomized clinical trial on treatment with Arnica montana to reduce post-operative seroma and bleeding in patients undergoing total mastectomy. J Intercult Ethnopharmacol 2017; 6: 1-8
- 11 Bagot JL, Delègue C. My best case: homeopathic management of adverse effects of tamoxifen. Wien Med Wochenschr 2020; 170: 224-229
- 12 Gaertner K, Lüer SC, Frei-Erb M, von Ammon K. Complementary individual homeopathy in paediatric cancer care: a case series from a University Hospital, Switzerland. Complement Ther Med 2018; 41: 267-270
- 13 Samuels N, Freed Y, Weitzen R. et al. Feasibility of homeopathic treatment for symptom reduction in an integrative oncology service. Integr Cancer Ther 2018; 17: 486-492
- 14 Rajendran ES. Homeopathy as a supportive therapy in cancer. Homeopathy 2004; 93: 99-102
- 15 Nwabudike LC. Homeopathy as therapy for mycosis fungoides: case reports of three patients. Homeopathy 2019; 108: 277-284
- 16 Bell IR, Sarter B, Koithan M. et al. Integrative nanomedicine: treating cancer with nanoscale natural products. Glob Adv Health Med 2014; 3: 36-53
- 17 Frenkel M. Is there a role for homeopathy in cancer care? Questions and challenges. Curr Oncol Rep 2015; 17: 43
- 18 Yadav R, Jee B, Rao KS. How homeopathic medicine works in cancer treatment: deep insight from clinical to experimental studies. J Exp Ther Oncol 2019; 13: 71-76
- 19 PRISMA. Transparent reporting of systematic reviews and meta-analyses. Available at: prisma-statement.org. Accessed April 4, 2020
- 20 Liberati A, Altman DG, Tetzlaff J. et al. The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate health care interventions: explanation and elaboration. PLoS Med 2009; 6: e1000100
- 21 Moher D, Liberati A, Tetzlaff J, Altman DG. PRISMA Group. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. PLoS Med 2009; 6: e1000097
- 22 MacLaughlin BW, Gutsmuths B, Pretner E. et al. Effects of homeopathic preparations on human prostate cancer growth in cellular and animal models. Integr Cancer Ther 2006; 5: 362-372
- 23 Ferrari de Andrade L, Mozeleski B, Leck AR. et al. Inhalation therapy with M1 inhibits experimental melanoma development and metastases in mice. Homeopathy 2016; 105: 109-118
- 24 Banerjee A, Pathak S, Biswas SJ. et al. Chelidonium majus 30C and 200C in induced hepato-toxicity in rats. Homeopathy 2010; 99: 167-176
- 25 Kumar KB, Sunila ES, Kuttan G, Preethi KC, Venugopal CN, Kuttan R. Inhibition of chemically induced carcinogenesis by drugs used in homeopathic medicine. Asian Pac J Cancer Prev 2007; 8: 98-102
- 26 Thangapazham RL, Rajeshkumar NV, Sharma A. et al. Effect of homeopathic treatment on gene expression in Copenhagen rat tumor tissues. Integr Cancer Ther 2006; 5: 350-355
- 27 Munshi R, Joshi S, Talele G, Shah R. An in-vitro assay estimating changes in melanin content of melanoma cells due to ultra-dilute, potentized preparations. Homeopathy 2019; 108: 183-187
- 28 Joshi S, Munshi R, Talele G, Shah R. Evaluation of melanogenic and anti-vitiligo activities of homeopathic preparations on murine B16-F10 melanoma cells. Eur J Pharm Med Res 2017; 4: 718-723
- 29 Klein SD, Würtenberger S, Wolf U, Baumgartner S, Tournier A. Physicochemical investigations of homeopathic preparations: a systematic review and bibliometric analysis – part 1. J Altern Complement Med 2018; 24: 409-421
- 30 Waisse S. Effects of high dilutions on in vitro models: literature review. Rev Homeopatia 2017; 80: 90-103
- 31 Gonçalves JP, Potrich FB, Ferreira Dos Santos ML. et al. In vitro attenuation of classic metastatic melanoma‑related features by highly diluted natural complexes: molecular and functional analyses. Int J Oncol 2019; 55: 721-732
- 32 Şeker S, Güven C, Akçakaya H, Bahtiyar N, Akbaş F, Onaran İ. Evidence that extreme dilutions of paclitaxel and docetaxel alter gene expression of in vitro breast cancer cells. Homeopathy 2018; 107: 32-39
- 33 Bellavite P, Signorini A, Marzotto M, Moratti E, Bonafini C, Olioso D. Cell sensitivity, non-linearity and inverse effects. Homeopathy 2015; 104: 139-160
- 34 Boericke W. Pocket manual of homoeopathic materia medica. New York, NY: Boericke & Runyon; 1922
- 35 Seligmann IC, Lima PDL, Cardoso PCS. et al. The anti-cancer homeopathic composite “Canova Method” is not genotoxic for human lymphocytes in vitro. Genet Mol Res 2003; 2: 223-228
- 36 Pathak S, Kumar Das J, Jyoti Biswas S, Khuda-Bukhsh AR. Protective potentials of a potentized homeopathic drug, Lycopodium-30, in ameliorating azo dye induced hepatocarcinogenesis in mice. Mol Cell Biochem 2006; 285: 121-131
- 37 Biswas SJ, Pathak S, Bhattacharjee N, Das JK, Khuda-Bukhsh AR. Efficacy of the potentized homeopathic drug, Carcinosin 200, fed alone and in combination with another drug, Chelidonium 200, in amelioration of p-dimethylaminoazobenzene-induced hepatocarcinogenesis in mice. J Altern Complement Med 2005; 11: 839-854
- 38 Biswas SJ, Khuda-Bukhsh AR. Evaluation of protective potentials of a potentized homeopathic drug, Chelidonium majus, during azo dye induced hepatocarcinogenesis in mice. Indian J Exp Biol 2004; 42: 698-714
- 39 Biswas SJ, Khuda-Bukhsh AR. Effect of a homeopathic drug, Chelidonium, in amelioration of p-DAB induced hepatocarcinogenesis in mice. BMC Complement Altern Med 2002; 2: 4
- 40 Khuda-Bukhsh AR, Mondal J, Shah R. Therapeutic potential of HIV nosode 30c as evaluated in A549 lung cancer cells. Homeopathy 2017; 106: 203-213
- 41 Mondal J, Samadder A, Khuda-Bukhsh AR. Psorinum 6x triggers apoptosis signals in human lung cancer cells. J Integr Med 2016; 14: 143-153
- 42 Sikdar S, Kumar Saha S, Rahman Khuda-Bukhsh A. Relative apoptosis-inducing potential of homeopathic Condurango 6c and 30c in H460 lung cancer cells in vitro: apoptosis-induction by homeopathic Condurango in H460 cells. J Pharmacopuncture 2014; 17: 59-69
- 43 Samadder A, Das S, Das J, Paul A, Boujedaini N, Khuda-Bukhsh AR. The potentized homeopathic drug, Lycopodium clavatum (5C and 15C) has anti-cancer effect on HeLa cells in vitro. J Acupunct Meridian Stud 2013; 6: 180-187
- 44 Bishayee K, Sikdar S, Khuda-Bukhsh AR. Evidence of an epigenetic modification in cell-cycle arrest caused by the use of ultra-highly-diluted Gonolobus condurango extract. J Pharmacopuncture 2013; 16: 7-13
- 45 Mukherjee A, Boujedaini N, Khuda-Bukhsh AR. Homeopathic Thuja 30C ameliorates benzo(a)pyrene-induced DNA damage, stress and viability of perfused lung cells of mice in vitro. J Integr Med 2013; 11: 397-404
- 46 Nascimento HFS, Cardoso PCDS, Ribeiro HF. et al. In vitro assessment of anticytotoxic and antigenotoxic effects of CANOVA(®). Homeopathy 2016; 105: 265-269
- 47 Champault G, Patel JC, Bonnard AM. A double-blind trial of an extract of the plant Serenoa repens in benign prostatic hyperplasia. Br J Clin Pharmacol 1984; 18: 461-462
- 48 Wani K, Shah N, Prabhune A, Jadhav A, Ranjekar P, Kaul-Ghanekar R. Evaluating the anti-cancer activity and nanoparticulate nature of homeopathic preparations of Terminalia chebula . Homeopathy 2016; 105: 318-326
- 49 Frenkel M, Mishra BM, Sen S. et al. Cytotoxic effects of ultra-diluted remedies on breast cancer cells. Int J Oncol 2010; 36: 395-403
- 50 Wälchli C, Baumgartner S, Bastide M. Effect of low doses and high homeopathic potencies in normal and cancerous human lymphocytes: an in vitro isopathic study. J Altern Complement Med 2006; 12: 421-427
- 51 Dalboni LC, Coelho CP, Palombo Pedro RR. et al. Biological actions, electrical conductance and silicon-containing microparticles of Arsenicum album prepared in plastic and glass vials. Homeopathy 2019; 108: 12-23
- 52 Demangeat JL. Gas nanobubbles and aqueous nanostructures: the crucial role of dynamization. Homeopathy 2015; 104: 101-115
- 53 Agarwal A, Ng WJ, Liu Y. Principle and applications of microbubble and nanobubble technology for water treatment. Chemosphere 2011; 84: 1175-1180
- 54 Agmon N, Bakker HJ, Campen RK. et al. Protons and hydroxide ions in aqueous systems. Chem Rev 2016; 116: 7642-7672
- 55 Yinnon TA. Liquids prepared by serially diluting and vigorously shaking of aqueous solutions: unveiling effects of the solute on their properties. Water 2020; 10: 115-134
- 56 Allen T. The Encyclopedia of Pure Materia Medica. New York, NY: Boericke & Tafel; 1877
- 57 Lima LF, Rocha RMP, Duarte ABG. et al. Unexpected effect of the vehicle (grain ethanol) of homeopathic FSH on the in vitro survival and development of isolated ovine preantral follicles. Microsc Res Tech 2017; 80: 406-418
- 58 Sandri PF, Portocarrero AR, Ciupa L. et al. Dynamized ethyl alcohol improves immune response and behavior in murine infection with Trypanosoma cruzi . Cytokine 2017; 99: 240-248
- 59 Nagai MY, Dalboni LC, Cardoso TN. et al. Effects of homeopathic phosphorus on encephalitozoon cuniculi-infected macrophages in vitro. Homeopathy 2019; 108: 188-200
- 60 Bonamin LV, Carvalho AC, Amaral J, Cardoso TN, Perez EC, Peres GB. Combination of homeopathic potencies, immune response and tumour microenvironment. In: Bonamin LV, Waisse S. eds. Transdisciplinarity and Translationality in High Dilution Research. (Signals and Images GIRI Series; ). Cambridge: Cambridge Scholars Publishing; 2019: 211-243
- 61 Stock-Schröer B. Reporting experiments in homeopathic basic research (REHBaR). Homeopathy 2015; 104: 333-336
- 62 Chirumbolo S, Bjørklund G. Homeopathic dilutions, Hahnemann principles, and the solvent issue: must we address ethanol as a “homeopathic” or a “chemical” issue?. Homeopathy 2018; 107: 40-44
- 63 de Oliveira CC, de Oliveira SM, Godoy LM, Gabardo J, Buchi Dde F. Canova, a Brazilian medical formulation, alters oxidative metabolism of mice macrophages. J Infect 2006; 52: 420-432
- 64 Arora S, Aggarwal A, Singla P, Jyoti S, Tandon S. Anti-proliferative effects of homeopathic medicines on human kidney, colon and breast cancer cells. Homeopathy 2013; 102: 274-282