Download PDF
CC BY-NC-ND 4.0 · J Lab Physicians 2020; 12(01): 32-37
DOI: 10.1055/s-0040-1715553
Original Article

Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation

Emsal Pinar Topdagi Yilmaz
1   Department of Gynecology and Obstetrics, Faculty of Medicine, Ataturk University, Erzurum, Turkey
,
Harun Un
2   Department of Biochemistry, Faculty of Pharmacy, Ağrı Ibrahim Cecen University, Agri, Turkey
,
Betul Gundogdu
3   Department of Pathology, Faculty of Medicine, Ataturk University, Erzurum, Turkey
,
Elif Polat
4   Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey
,
Seda Askin
4   Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey
,
Yunus Emre Topdagi
5   Department of Gynecology and Obstetrics, Faculty of Medicine, Sanko University, Gaziantep, Turkey
,
Zekai Halici
6   Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey
› Author Affiliations Funding The authors declared that this study received no financial support.
› Further Information
   Permissions and Reprints

Abstract

Objective The aim of our study was to evaluate the effect of two different doses of lycopene, an antioxidant, on experimentally induced ovarian ischemia/reperfusion (IR) injury in rat model.

Materials and Methods Twenty-four female rats were randomly divided into four groups: sham operation (group 1), 3-hour ischemia, 3-hour reperfusion (IR) (group 2), and IR + 100 mg/kg lycopene (PO) (group 3), IR + 200 mg/kg of lycopene (group 4). The rats’ superoxide dismutase (SOD), myeloperoxidase (MPO) activities, malondialdehyde (MDA), and glutathione (GSH) levels were calculated. Ovarian tissue damage was assessed using a histopathological scoring system.

Results Serum parameter levels and histological scores showed that treatment with lycopene may be conservative approach to prevent IR injury after the ovarian detorsion procedure.The improvement with lycopene was higher at 200 mg than at 100 mg. The MPO and MDA values were significantly lower in groups 3 and 4 as compared with group 2 (p < 0.05), whereas the MPO and MDA values were lower in group 4 as compared with group 3.The SOD and GSH values were significantly higher in groups 3 and 4 as compared with group 2 (p < 0.05), whereas the SOD and GSH values were higher in group 4 as compared with group 3.Tissue damage scores were elevated in the IR group compared with the sham group, but the treatment with different lycopene doses after reperfusion improved the histopathological tissue damage scores.

Conclusion The results showed that lycopene treatment reduced ovarian IR damage. Antioxidant activity was found to increase in a dose-dependent manner. Lycopene treatment may be conservative approach for ovarian torsion patients after the detorsion procedure to prevent IR damage.

Keywords

ischemia/reperfusion - lycopene - ovarian torsion - oxidative stress


Publication History

Article published online:
11 August 2020

© .

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

  • References

  • 1 Bayir Y, Cadirci E, Polat B. et al. Aliskiren - a promising strategy for ovarian ischemia/reperfusion injury protection in rats via RAAS. Gynecol Endocrinol 2016; 32 (08) 675-683
    CrossrefPubMedGoogle Scholar
  • 2 Oral A, Halici Z, Bayir Y. et al. Effects of oral zinc administration on long-term ipsilateral and contralateral testes damage after experimental testis ischaemia-reperfusion. Andrologia 2017; 49 (06) e12673
    CrossrefPubMedGoogle Scholar
  • 3 Sahin K, Yenice E, Tuzcu M. et al. Lycopene protects against spontaneous ovarian cancer formation in laying hens. J Cancer Prev 2018; 23 (01) 25-36
    CrossrefPubMedGoogle Scholar
  • 4 Oral A, Odabasoglu F, Halici Z. et al. Protective effects of montelukast on ischemia-reperfusion injury in rat ovaries subjected to torsion and detorsion: biochemical and histopathologic evaluation. Fertil Steril 2011; 95 (04) 1360-1366
    CrossrefPubMedGoogle Scholar
  • 5 Yigiter M, Halici Z, Odabasoglu F. et al. Growth hormone reduces tissue damage in rat ovaries subjected to torsion and detorsion: biochemical and histopathologic evaluation. Eur J Obstet Gynecol Reprod Biol 2011; 157 (01) 94-100
    CrossrefPubMedGoogle Scholar
  • 6 Cakir Gungor AN, Gencer M, Karaca T. et al. The effect of hesperetin on ischemia-reperfusion injury in rat ovary. Arch Gynecol Obstet 2014; 290 (04) 763-769
    CrossrefPubMedGoogle Scholar
  • 7 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254
    CrossrefPubMedGoogle Scholar
  • 8 Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95 (02) 351-358
    CrossrefPubMedGoogle Scholar
  • 9 Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol 1982; 78 (03) 206-209
    CrossrefPubMedGoogle Scholar
  • 10 Eser A, Hizli D, Haltas H. et al. Effects of curcumin on ovarian ischemia-reperfusion injury in a rat model. Biomed Rep 2015; 3 (06) 807-813
    CrossrefPubMedGoogle Scholar
  • 11 Halici Z, Karaca M, Keles ON. et al. Protective effects of amlodipine on ischemia-reperfusion injury of rat ovary: biochemical and histopathologic evaluation. Fertil Steril 2008; 90 (06) 2408-2415
    CrossrefPubMedGoogle Scholar
  • 12 Zimmerman BJ, Granger DN. Reperfusion injury. Surg Clin North Am 1992; 72 (01) 65-83
    CrossrefPubMedGoogle Scholar
  • 13 Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury. J Pathol 2000; 190 (03) 255-266
    CrossrefPubMedGoogle Scholar
  • 14 Un H, Bayir Y, Halici Z. et al. The effects of RAAS Inhibition in rate limiting step by Aliskiren on testicular torsion injury in rats. J Urol 2015; 194 (03) 828-833
    CrossrefPubMedGoogle Scholar
  • 15 Ece A, Kelekçi S, Hekimoğlu A. et al. Neutrophil activation, protein oxidation and ceruloplasmin levels in children with Henoch-Schönlein purpura. Pediatr Nephrol 2007; 22 (08) 1151-1157
    CrossrefPubMedGoogle Scholar
  • 16 Kaya C, Karabulut R, Turkyilmaz Z. et al. Lycopene has reduced renal damage histopathologically and biochemically in experimental renal ischemia-reperfusion injury. Ren Fail 2015; 37 (08) 1390-1395
    CrossrefPubMedGoogle Scholar
  • 17 Dogan C, Halici Z, Topcu A. et al. Effects of amlodipine on ischaemia/reperfusion injury in the rat testis. Andrologia 2016; 48 (04) 441-452
    CrossrefPubMedGoogle Scholar
  • 18 Cadirci E, Oral A, Odabasoglu F. et al. Atorvastatin reduces tissue damage in rat ovaries subjected to torsion and detorsion: biochemical and histopathologic evaluation. Naunyn Schmiedebergs Arch Pharmacol 2010; 381 (05) 455-466
    CrossrefPubMedGoogle Scholar
  • 19 Halestrap AP, Clarke SJ, Javadov SA. Mitochondrial permeability transition pore opening during myocardial reperfusion–a target for cardioprotection. Cardiovasc Res 2004; 61 (03) 372-385
    CrossrefPubMedGoogle Scholar
  • 20 Akgür FM, Kilinç K, Aktuğ T. Reperfusion injury after detorsion of unilateral testicular torsion. Urol Res 1993; 21 (06) 395-399
    CrossrefPubMedGoogle Scholar
  • 21 Kisaoglu A, Borekci B, Yapca OE, Bilen H, Suleyman H. Tissue damage and oxidant/antioxidant balance. Eurasian J Med 2013; 45 (01) 47-49
    CrossrefPubMedGoogle Scholar
  • 22 Sağsöz N, Kisa U, Apan A. Ischaemia-reperfusion injury of rat ovary and the effects of vitamin C, mannitol and verapamil. Hum Reprod 2002; 17 (11) 2972-2976
    CrossrefPubMedGoogle Scholar
  • 23 Yildirim N, Simsek D, Kose S. et al. The protective effect of Gingko biloba in a rat model of ovarian ischemia/reperfusion injury: improvement in histological and biochemical parameters. Adv Clin Exp Med 2018; 27 (05) 591-597
    CrossrefPubMedGoogle Scholar
  • 24 Kawata A, Murakami Y, Suzuki S, Fujisawa S. Anti-inflammatory activity ofβ-carotene, lycopene and tri-n-butylborane, a scavenger of reactive oxygen species. In Vivo 2018; 32 (02) 255-264
    PubMedGoogle Scholar
  • 25 Basu A, Imrhan V. Tomatoes versus lycopene in oxidative stress and carcinogenesis: conclusions from clinical trials. Eur J Clin Nutr 2007; 61 (03) 295-303
    CrossrefPubMedGoogle Scholar
  • 26 Hedayati N, Naeini MB, Nezami A. et al. Protective effect of lycopene against chemical and natural toxins: a review. Biofactors 2019; 45 (01) 5-23
    CrossrefPubMedGoogle Scholar
  • 27 Larson AM, Polson J, Fontana RJ. et al. Acute Liver Failure Study Group. Acetaminophen-induced acute liver failure: results of a United States multicenter, prospective study. Hepatology 2005; 42 (06) 1364-1372
    CrossrefPubMedGoogle Scholar
  • 28 Bandeira ACB, da Silva RC, Rossoni JV. et al. Lycopene pretreatment improves hepatotoxicity induced by acetaminophen in C57BL/6 mice. Bioorg Med Chem 2017; 25 (03) 1057-1065
    CrossrefPubMedGoogle Scholar
  • 29 Brattin WJ, Glende Jr EA, Recknagel RO. Pathological mechanisms in carbon tetrachloride hepatotoxicity. J Free Radic Biol Med 1985; 1 (01) 27-38
    CrossrefPubMedGoogle Scholar
  • 30 Pinto C, Rodriguez-Galdon B, Cestero JJ, Macias P. Hepatoprotective effects of lycopene against carbon tetrachloride-induced acute liver injury in rats. J Funct Foods 2013; 5 (04) 1601-1610
    CrossrefGoogle Scholar
  • 31 Walker PD, Barri Y, Shah SV. Oxidant mechanisms in gentamicin nephrotoxicity. Ren Fail 1999; 21 (3-4) 433-442
    CrossrefPubMedGoogle Scholar
  • 32 Karahan I, Ateşşahin A, Yilmaz S, Ceribaşi AO, Sakin F. Protective effect of lycopene on gentamicin-induced oxidative stress and nephrotoxicity in rats. Toxicology 2005; 215 (03) 198-204
    CrossrefPubMedGoogle Scholar
  • 33 Cohen DJ, Loertscher R, Rubin MF, Tilney NL, Carpenter CB, Strom TB. Cyclosporine: a new immunosuppressive agent for organ transplantation. Ann Intern Med 1984; 101 (05) 667-682
    CrossrefPubMedGoogle Scholar
  • 34 Ateşşahin A, Ceribaşi AO, Yilmaz S. Lycopene, a carotenoid, attenuates cyclosporine-induced renal dysfunction and oxidative stress in rats. Basic Clin Pharmacol Toxicol 2007; 100 (06) 372-376
    CrossrefPubMedGoogle Scholar
  • 35 Liu X, Lin X, Zhang S. et al. Lycopene ameliorates oxidative stress in the aging chicken ovary via activation of Nrf2/HO-1 pathway. Aging (Albany NY) 2018; 10 (08) 2016-2036
    CrossrefPubMedGoogle Scholar
  • 36 Celik M, Aksoy AN, Aksoy H, Aksoy Y, Halici Z. Sildenafil reduces ischemia-reperfusion injury in rat ovary: biochemical and histopathological evaluation. Gynecol Obstet Invest 2014; 78 (03) 162-167
    CrossrefPubMedGoogle Scholar
  • 37 Erten SF, Kocak A, Ozdemir I, Aydemir S, Colak A, Reeder BS. Protective effect of melatonin on experimental spinal cord ischemia. Spinal Cord 2003; 41 (10) 533-538
    CrossrefPubMedGoogle Scholar
  • 38 Tok A, Sener E, Albayrak A. et al. Effect of mirtazapine on oxidative stress created in rat kidneys by ischemia-reperfusion. Ren Fail 2012; 34 (01) 103-110
    CrossrefPubMedGoogle Scholar
  • 39 Sayar I, Bicer S, Gursul C, Gürbüzel M, Peker K, Işik A. Protective effects of ellagic acid and ozone on rat ovaries with an ischemia/reperfusion injury. J Obstet Gynaecol Res 2016; 42 (01) 52-58
    CrossrefPubMedGoogle Scholar
  • 40 Yapca OE, Kumbasar S, Salman S. et al. Controlled reperfusion for different durations in the treatment of ischemia-reperfusion injury of the rat ovary: evaluation of biochemical features, molecular gene expression, and histopathology. Can J Physiol Pharmacol 2015; 93 (04) 269-274
    CrossrefPubMedGoogle Scholar