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CC BY-NC-ND 4.0 · Eur J Dent 2020; 14(03): 410-414
DOI: 10.1055/s-0040-1712776
Original Article

The Macrophage Responses during Diabetic Oral Ulcer Healing by Liquid Coconut Shell Smoke: An Immunohistochemical Analysis

Meircurius Dwi Condro Surboyo 
1   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Fatma Yasmin Mahdani
1   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Diah Savitri Ernawati
1   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Andari Sarasati
2   Department of Dental Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Fianza Rezkita
2   Department of Dental Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
› Author Affiliations Funding This study was funded by the Faculty of Dental Medicine - Universitas Airlangga, Surabaya, Indonesia 2019, under grant 1408/UN3/2019.
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Abstract

Objectives Liquid coconut shell smoke (LC-SS) is used in natural food preservation for a long history. The purpose of this study was to analyze the role of LC-SS in macrophage responses during diabetic oral ulcer healing as medication.

Materials and Methods Oral ulcers were induced in the labial lower mucosa of the research subjects using a round steel blade following diabetic induction by means of alloxan. Twenty-four diabetic Wistar rats presenting oral ulcers were divided into two groups, a test group, which was given topical treatment of LC-SS and a control group, which was given benzydamine hydrochloride (BHCl). The role of LC-SS in macrophages was assessed by means of immunohistochemistry for nuclear factor kappa B (NF-κB) and tumor necrosis factor-α (TNF-α) expression.

Result LC-SS increased macrophages compared with BHCl (p = 0.000). The LC-SS affected only TNF-α expression by stimulating NF-κB expression (p = 0.046) but did not macrophage numbers (p = 0.861).

Conclusion LC-SS has a stronger effect compared with BHCl on diabetic oral ulcer healing by increasing macrophage response to produce TNF-α while decreasing NF-κB expression.

Keywords

macrophages - liquid smoke - benzydamine hydrochloride - oral ulcer - coconut shell


Publication History

Article published online:
24 May 2020

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Thieme Medical and Scientific Publishers Private Ltd.
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