Preparation and Standardization of Escherichia coli Nosodes Sourced from Select E. coli Strains
17 September 2019
04 February 2020
29 July 2020 (online)
Background The nosodes are well-known preparations in homeopathy that are sourced from organisms and diseased materials. More than 40 known nosodes have been used in homeopathic practice for over a century. Having identified the need for scientifically developed new nosodes sourced from organisms that are currently prevalent, the preparation of Escherichia coli nosodes from different strains of the bacterium is presented in this article.
Materials and Methods Escherichia coli strains (E. coli ATCC 11775E, ATCC 25922, and ATCC 8739) were identified, cultured, and tested for purity, and 20 billion cells were processed following the nosode preparation method given in the Homoeopathic Pharmacopoeia of India, group N1. Serial dilution and potentization for liquid potency were done up to 30c potency. Nosodes were prepared by two methods: from cell-free extract (endotoxin) and from entire-cell extract.
Result Six nosodes were developed in total. Three univalent nosodes were prepared using individual endotoxins, one from each of the three E. coli strains; those three univalent nosodes were also combined as “Trivalent nosode-I”. “Trivalent nosode-II” was prepared by mixing entire cells of the three E. coli strains. A mix of both Trivalent nosode-I and Trivalent nosode-II was labeled “EC-Polynosode”. The safety profile of the potentized nosodes was documented by the non-detectability of traces of source material (absence of contamination, live organisms, or DNA material) through a culture test, sterility test, and molecular testing (polymerase chain reaction).
Conclusion Different variants of E. coli nosodes were systematically and scientifically prepared and standardized using the cultures. Homeopathic pathogenetic trials, in-vitro efficacy studies, and clinical evaluation of E. coli nosodes (single, trivalent, or polyvalent nosodes) will be required in future.
• There is a recognized need for the scientific development of nosodes from currently prevalent organisms.
• Strains of E. coli (ATCC 11775E, ATCC 25922 and ATCC 8739) and their endotoxins were selected and standardized for preparing E. coli nosodes, using single strains and their combinations.
• The safety of E. coli nosodes using culture, sterility, and molecular tests was established.
• These E. coli nosodes can be explored in pre-clinical and clinical efficacy evaluation.
Renuka Munshi developed the laboratory facility, conducted the research, and reviewed the manuscript. Gitanjali Talele contributed to laboratory work and co-wrote the manuscript. Rajesh Shah is the principal investigator, who developed the drug, conceptualized the study, supervised the laboratory work, and co-wrote the manuscript.
- 1 World Health Organization. E. coli key facts. Available at: https://www.who.int/news-room/fact-sheets/detail/e-coli . Accessed September 4, 2019
- 2 Irino K, Vaz TM, Kato MA. , et al. O157:H7 Shiga toxin-producing Escherichia coli strains associated with sporadic cases of diarrhea in São Paulo, Brazil. Emerg Infect Dis 2002; 8: 446-447
- 3 European Center for Disease Prevention and Control. Facts about Escherichia coli. Available at: https://ecdc.europa.eu/en/escherichia-coli-ecoli/facts . Accessed August 22, 2019
- 4 Homoeopathic Pharmacopoeia of India. 1st ed. Vol IV. New Delhi: Ministry of Health Government of India; 1983: 136-137
- 5 Allen HC. The Materia Medica of the Nosodes. With Provings of the X-Ray. New Delhi: B Jain Publishers Pvt. Ltd; 1988
- 6 Shah R. Clinical trial for evaluation of a human immunodeficiency virus nosode in the treatment for human immunodeficiency virus-infected individuals. Indian J Res Homoeopathy. 2015; 9: 25-33
- 7 Shah R. Hepatitis C nosode: the preparation and homeopathic pathogenetic trial. Homeopathy 2013; 102: 207-214
- 8 Joshi S, Mukerjee S, Vaidya S, Talele G, Chowdhary A, Shah R. Preparation, standardization and in vitro safety testing of Mycobacterium nosodes (Emtact- polyvalent nosode). Homeopathy 2016; 105: 225-232
- 9 Joshi S, Munshi R, Talele G, Shah R. An experimental in vitro study to evaluate the antimalarial activity of select homeopathy preparations. Int J Med Health Res 2017; 7: 65-68
- 10 Shah R, Talele G. Preparation of cancer nosodes from specific cancer tissues. Int J High Dilution Res 2019; 18: 28-34
- 11 Allen HC. The Materia Medica of the Nosodes. With Provings of the X-Ray. New Delhi: B Jain Publishers Pvt. Ltd; 1988: 544
- 12 Bacillus Coli (Colibacillinum) CH description. Available at: https://www.schwabeindia.com/2717-colibacillinum.html . Accessed September 4, 2019
- 13 Julian OA. Materia Medica of Nosodes with Repertory – Treatise on Dynamised Micro-immunotherapy, Part II. Materia Medica of Biotherapic Nosode. New Delhi: B. Jain Publishers; 2003: 202
- 14 Madrewar BP. Therapeutics of Veterinary Homeopathy. New Delhi: B. Jain Publishers; 2004
- 15 Dominici G, Allegri F, Andreotti MC. , et al. Proving of Colibacillinum. Complete Repertory 2019, Repertory by Roger Zandvoort. Available at: https://hompath.com/complete-repertory-2019 . Accessed August 20, 2019
- 16 Riley DS. Bacterium coli. In: Materia Medica of New and Old Homeopathic Medicines. Chapter 13. Berlin, Heidelberg: Springer; 2012: 37-39
- 17 Camerlink I, Ellinger L, Bakker EJ, Lantinga EA. Homeopathy as replacement to antibiotics in the case of Escherichia coli diarrhoea in neonatal piglets. Homeopathy 2010; 99: 57-62
- 18 Miko A, Rivas M, Bentancor A, Delannoy S, Fach P, Beutin L. Emerging types of Shiga toxin-producing E. coli (STEC) O178 present in cattle, deer, and humans from Argentina and Germany. Front Cell Infect Microbiol 2014; 4: 78
- 19 Extended-spectrum beta-lactamases (or ESBLs). Available at: https://www.healthline.com/health/esbl
- 20 Shah R. Standardization of the potentizing machine and quantification of the impact of potentization. Indian J Res Homoeopathy. 2016; 10: 126-132 . Accessed September 16, 2019
- 21 Indian Pharmacopoeia. New Delhi: The Indian Pharmacopoeia Commission Ministry of Health and Family Welfare, Government of India; 2010: 57-58
- 22 Sarowska J, Futoma-Koloch B, Jama-Kmiecik A. , et al. Virulence factors, prevalence and potential transmission of extraintestinal pathogenic Escherichia coli isolated from different sources: recent reports. Gut Pathog 2019; 11: 10
- 23 Shah R. Scientific method of preparing homeopathic nosodes. Indian J Res Homoeopathy 2014; 8: 166-174
- 24 Shah R. Evaluating the anticancer effects of high-dilution preparations of carcinogens such as HIV virus, Hepatitis C virus, ethanol and cancer tissues in in-vitro models. Int J High Dilution Res. 2019; 18: 12-27