CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2017; 07(04): 011-015
DOI: 10.1055/s-0040-1709243
Original Article

Comparative Evaluation of Pre-Donation Haemoglobin Screening Methods

Chandrika Rao
1   Assistant Professor and Blood Bank Officer, Department of Pathology, Mangalore
Sanjana D. S.
2   MBBS Student, K S Hegde Medical Academy Derlakatte, Mangalore
› Author Affiliations


Background:The pre-donation haemoglobin (Hb) estimation is the only laboratory test done on blood donors to determine an individual's eligibility to donate blood with an intention to prevent bleeding an anaemic donor. With availability of wide range of screening methods, no single technique has emerged as the most appropriate and ideal for screening blood donors. The primary objective of the study was to compare results of copper sulphate method and haemoglobin meter with the gold standard Cyanmeth hemoglobin (CyanHb) method.

Materials and methods : Prospective observational study done in 238 blood donors. Sample analyses were done using Copper sulphate solution, haemoglobin meter and Cyanmeth haemoglobin method.

Results : Mean values of three methods Cyanmeth haemoglobin, and Haemoglobin meter methods are 12.5±2.2, and 13.08±1.61 respectively. Haemoglobin values obtained by haemoglobin meter and copper sulphate methods were comparable, with sensitivity of 94.25% and 94.47% respectively.

Conclusion : CuSo4 method and haemoglobin meter can be used alternatively based on resources available. The higher values obtained by CyanHb method could be due to turbidity factor of the lipids in the blood sample.

Publication History

Received: 12 March 2017

Accepted: 12 August 2017

Article published online:
21 April 2020

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