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Evaluation of Histological Properties of Human Meniscal Grafts Stored in a Tissue Bank[∗]Article in several languages: português | English
Objectives The present paper aims to evaluate and compare the histological features of fresh and frozen menisci stored in a tissue bank for 1 month and for 5 years.
Methods The meniscal grafts were subjected to a histological study. A total of 10 menisci were evaluated; 2 were frozen for 5 years, 4 were frozen for 1 month, and 4 were fresh, recently harvested specimens. Histological properties were evaluated in sections stained with hematoxylin and eosin and Masson trichrome methods.
Results The menisci frozen for 1 month showed partially preserved collagen fiber structure and no significant hydropic tissue degeneration. The menisci frozen for 5 years presented an evident dissociation of collagen fibers and multiple foci of hydropic degeneration.
Discussion Degeneration was much more significant in menisci stored for 5 years, indicating that a long freezing period results in substantial progression of tissue deterioration. This may suggest that the 5-year period, considered the maximum time for graft storage before transplant, is too long.
Conclusion Grafts stored for 1 month showed a slight degenerative change in collagen fibers, whereas menisci frozen for 5 years presented significant tissue degeneration.
∗ Study performed by the Knee Group from the Departamento de Ortopedia e Traumatologia, Irmandade da Santa Casa de Misericórdia de São Paulo - “Pavilhão Fernandinho Simonsen”, São Paulo, SP, Brazil.
• Netto A. S. – Study conceptualization and design, data analysis and interpretation, manuscript preparation.
• Antebi U. – Data analysis and interpretation.
• Morais C. E.– Data analysis and interpretation.
• Sementilli L. – Data analysis and interpretation.
• Severino N. R. – Critical review of the article.
• Cury R. P. L. – Critical review of the article and approval of the final version for publication.
Received: 30 July 2019
Accepted: 10 January 2020
08 June 2020 (online)
© 2020. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)
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