CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2017; 07(03): 042-048
DOI: 10.1055/s-0040-1708723
Review Article

Loop-mediated isothermal amplification (LAMP) : A rapid molecular diagnosis technique for detection of human pathogens

Gunimala Chakraborty
1  DST women scientist, Division of Infectious Diseases, Nitte University Centre for Science Education and Research Paneer Campus, Nitte University, Mangalore, Karnataka, India.
,
Indrani Karunasagar
2  Director (R&D), Dean, Faculty of Biological Sciences, Nitte University, Mangalore, Karnataka, India.
,
Anirban Chakraborty
3  Deputy Director, Nitte University Centre for Science Education and Research, Nitte University, Mangalore, Karnataka, India.
› Author Affiliations

Abstract

Delivery of quality healthcare in case of an infectious disease depends on how efficiently and how quickly the responsible pathogens are detected from the samples. Molecular methods can detect the presence of pathogens in a rapid and sensitive manner. Over the years, a number of such assays have been developed. However, these methods, although highly reliable and efficient, require use of expensive equipment, reagents, and trained personnel. Therefore, development of molecular assays that are simple, rapid, cost-effective, yet sensitive, is highly warranted to ensure efficient management or treatment strategies. Loop-mediated isothermal amplification (LAMP), a technique invented in the year 2000, is a novel method that amplifies DNA at isothermal conditions. Since its invention, this technique has been one of the most extensively used molecular diagnostic tools in the field of diagnostics offering rapid, accurate and cost-effective diagnosis of infectious diseases. Using the LAMP principle, many commercial kits have been developed in the last decade for a variety of human pathogens including bacteria, viruses and parasites. Currently LAMP assay is being considered as an effective diagnostic tool for use in developing countries because of its simple working protocol, allowing even an onsite application. The focus of this review is to describe the salient features of this technique the current status of development of LAMP assays with an emphasis on the pathogens of clinical significance.



Publication History

Received: 27 March 2017

Accepted: 23 August 2017

Publication Date:
22 April 2020 (online)

© .

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 
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