CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2014; 04(04): 076-080
DOI: 10.1055/s-0040-1703837
Original Article

EVALUATION OF ANTIMICROBIAL AND CYTOTOXIC ACTIVITY OF ELECTRON BEAM IRRADIATED ENDODONTIC SEALER

A Geethashri
1  Senior Research Fellow, Nitte University Centre for Science Education & Research, Mangalore University, Mangalore, India
,
KJ Palaksha
2  Associate Professor, Nitte 5 University Centre for Stem Cell Research & Regenerative Medicine, Mangalore University, Mangalore, India
,
B Mohana Kumar
2  Associate Professor, Nitte 5 University Centre for Stem Cell Research & Regenerative Medicine, Mangalore University, Mangalore, India
,
KR Sridhar
3  Professor & HOD, Department of Biosciences, Mangalore University, Mangalore, India
,
Ganesh Sanjeev
4  Senior Physicist, Mangalore University, Mangalore, India
,
A Veena Shetty
5  Associate Professor, Department of Microbiology, K.S. Hegde Medical Academy, Nitte University,Mangalore University, Mangalore, India
› Author Affiliations

Abstract

Background: The persistent pathogenic microorganisms in root canal system even after chemo-mechanical preparations cause endodontic infection and failure of the treatment. Thus the filling material, in addition to its good sealing ability, should offer long term antimicrobial effect and be non-toxic to cells and dentin. Zinc Oxide Eugenol (ZOE) is the most commonly used root canal sealer in endodontics. Electron beam (e-beam) is an ionizing radiation and known to cause physiochemical and biological changes. The aim of this study was to evaluate the effect of e-beam irradiation on bioactive properties of ZOE.

Methodology: The homogenous mixture of ZOE was prepared as per manufacturer's instructions and discs of 6 mm were prepared by loading the paste into sterile moulds. After complete drying discs were aseptically removed and subjected to e-beam irradiation at doses of 250 Gy, 500 Gy, 750 Gy and 1000 Gy at Microtron Centre, Mangalore University. Antimicrobial and antibiofilm properties of both control(non-irradiated) and irradiated sealer against Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans and Candida albicans were determined by well diffusion method and antibiofilm by O'Toole method, respectively. The cytotoxicity was determined by using MTT assay on human gingival fibroblasts.

Results: The antimicrobial effect of ZOE was observed only against S. aureus and C. albicans. The ZOE sealer irradiated at 1000 Gy showed a significantly (P< 0.001) increased antimicrobial effect against S. aureus and C. albicans compared to control ZOE. However, the substantially increased antibiofilm activity against C. albicans was noticed in the ZOE irradiated at 250 Gy. There was no significant (P>0.05) difference in cytotoxicity between control and irradiated ZOE.

Conclusion: The e-beam irradiated endodontic sealer ZOE at 1000 Gy and 250 Gy significantly enhanced the antimicrobial and antibiofilm activity respectively without changing its biocompatibility.



Publication History

Publication Date:
26 April 2020 (online)

© .

Thieme Medical and Scientific Publishers Private Ltd.
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  • References

  • 1 Sandqvist G. Ecology of the root canal flora. J Endod 1992;18:427-430.
  • 2 D'Souza L Henston, Sharma N, Chander S, Singh S, D'Souza R. Root canal sealers and its role in successful endodontics- A review. Annals of Dental Research 2012;2(2):68-78.
  • 3 Zarrabi MH, Javidi M, Naderinasab M, Gharechahi M. comparative evaluation of antimicrobial activity of three cements: new endodontic cement (NEC), mineral trioxide aggregate (MTA) and portland. Journal of Oral Science 2009;51(3):437-442.
  • 4 Gomes BPFA, Pedroso JA, Jacinto RC, Visnns ME, Ferraz CCR, Zaia AA,. In vitro Evaluation of the Antimicrobial Activity of Five Root Canal Sealers. Braz Dent J 2004;15(1):30-35.
  • 5 Queiroz AM, Nelson-Filho P, Silva LAB, Assed S, Silva RAB, ITO IY. Antimicrobial Activity of Root Canal Filling Materials for Primary Teeth: Zinc oxide and eugenol Cement, Calen Paste Thickened with Zinc Oxide, Sealapex and EndoREZ. Braz Dent J 2009;20(4):290-296.
  • 6 Pizzo G, Giammanco GM, Cumbo E, Nicolosi G, Gallina G. In vitro antimicrobial activity of endodontic sealers. Jornal of Dentistry 2006;34:35-40.
  • 7 Beteshobabrud R, Nabardi F. The stability studies of penicillin and ampicillin following ?-irradiation in the solid state. Iranian Journal of Pharmaceutical Research 2009;8(3):153-157.
  • 8 Romanelli MF, Moraes MCF, Villavicencio ALCH, Borrely SI. Evaluation of toxicity reduction of sodium dodecyl sulfate submitted to electron beam irradiation. Radiation Phys. Chem. 2004:71;409-411.
  • 9 Narayanan LL, Vaiahnavi C. Endodontic Microbiology. J Conserv Dent 2010;13(2):233-239.
  • 10 Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic and apical periodontitis. Int Endod J 2001;34(6):429-34.
  • 11 Filho MT, Tonomaru JMG, Barros DB, Watanabe E, Ito IY. In vitro antimicrobial activity of endodontic sealers, MTA-based cements and Portland cement. J Oral Sci. 2007;49:41-45.
  • 12 O'Toole G.A. Microtiter Dish Biofilm Formation Assay. J Vis Exp. 2011; 30(47):1-2.
  • 13 Sergeant JM, Taylor CG. Appraisal of the MTT assay as a rapid test of chemosensitivity in acute myeloid leukaemia. Br. J.Cancer 1989; 60:206-210.
  • 14 Kaiwar A, Nadig G, Hegde J, Lekha S. Assessment of antimicroibial sealers on Enterococcu faecalis: An in vitro study. World Journal of Dentistry 2012;13(1):26-31.
  • 15 Hume WR. The pharmacological and toxicological properties of zinc oxide- eugenol. Journal of American Dental Association 1986;113:789-91.
  • 16 Lindqvist L, Otteskog O. Eugenol- liberation from the dental materials and effect on human diploid fibroblast cells. Scandinavian Journal of Dental Research 1981;89:552-556.
  • 17 and Cephalosporines: Assessment of radiation Damage on Antibiotics by changes in optical property and colorimetric parameters AAPS PharmSciTech 2009;10(1):34-43.