CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2014; 04(04): 004-009
DOI: 10.1055/s-0040-1703823
Original Article

PHYTOCHEMICAL AND ANTIMICROBIAL ACTIVITY OF THE LEAVES OF LANNEA KERSTINGII ENGL & K. KRAUSE (ANACADIACEAE)

Njinga NS
1  Lecturers, Department of Pharmaceutical & Medicinal Chemistry, University of Ilorin, Nigeria.
,
Sule MI
2  Professor, Ahmadu Bello University, Zaria, Nigeria.
,
Pateh UU
3  Associate Professor & Dean, Faculty of Pharmaceutical 5 Sciences, Ahmadu Bello University, Zaria, Nigeria.
,
Hassan HS
4  Senior Lecturer, Department of Pharmaceutical & Medicinal Chemistry, Ahmadu Bello University, Zaria, Nigeria.
,
Ahmad MM
5  Pharmacist, Dr. Gwamna Awan Memorial Hospital, Kakuri, Nigeria.
,
Abdullahi ST
6  Lecturers, Department of Pharmaceutical & Medicinal Chemistry, University of Ilorin, Nigeria.
,
Danja BA
7  Lecturer, Department of Chemical Sciences, Faculty of Science, Federal University Kashere, Gombe, Nigeria.
,
B Bawa
8  Researche Scholar, National Agency for Food and Drugs Administration and Control (NAFDAC), Nigeria.
› Author Affiliations

Abstract

The phytochemical and antimicrobial activity of the petroleum ether and crude methanol extracts, chloroform and ethyl acetate fractions of the leaves of Lannea kerstingii were investigated. Phytochemical screening revealed the presence of steroids and triterpenes in the petroleum ether extract, steroid, triterpene, flavonoids and tannins in both crude methanol extract and chloroform fraction while the ethyl acetate fraction contained only flavonoids and tannins. The extracts exhibited antimicrobial activities with zones of inhibition ranging from 17.00 to 21.03, 20.10 to 25.24, 25.32 to 34.02 and 22.28 to 27.20 mm for petroleum ether extract, methanol extract, chloroform and ethyl acetate fractions respectively. The minimum inhibitory concentration was between 5 and 10mg/ml, 5mg/ml for the petroleum ether and methanol extract respectively, and between 2.5 and 5 mg/ml, 5mg/ml for the acetate fractions. The minimum bactericidal concentration for all the extracts was 40mg/ml respectively except for chloroform fraction which ranged from 20 to 40mg/ml. The minimum fungicidal concentration for all the extracts was found to be 40mg/ml respectively. This result indicates the broad spectrum antimicrobial potential of L. Kerstingii and justifies the use of this plant in traditional medicine.



Publication History

Publication Date:
26 April 2020 (online)

© .

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 
  • References

  • 1 Lesney MS. Nature's Pharmaceuticals: Natural Products from Plants Remain at the Core of Modern Medicinal Chemistry. Today's Chemist at Work. 2004;13(7):26-31.
  • 2 Sharma A, Shanker C, Tyagi L, Singh M, Rao CV. Herbal Medicine for Market Potential in India: An Overview. Int. J. Plant Sci. 2008;1:26-36.
  • 3 Nair R, Chanda S. Antibacterial activities of some medicinal plants of the Western region of India. Turkish J. Biol. 2007;31:231-236.
  • 4 Longo DL, Kasper DL, Jameson JL, Fauci AS, Hauser SL, Loscalzo J. Harrison's Principles of Internal Medicine 18th edn. 2012; The McGraw-Hill Companies Inc, New York.
  • 5 Norrby RS, Nord CE, Finch R. Lack of development of new antimicrobial drugs: a potential serious threat to public health. Lancet. 2005;5:115-119.
  • 6 Sung WS, Lee DG. In vitro antimicrobial activity and the mode of action of indol-3-carbinol against human pathogenic microorganisms. Biol. Pharm. Bull. 2007;30:1865-1869.
  • 7 Arbonnier M. Trees, shrubs and lianas of West African dry zones. 2002; CIRAD, MNHN
  • 8 Doka IG, Yagi SM. Ethnobotanical Survey of Medicinal Plants in West Kordofan (Western Sudan). Ethnobot Leaflets. 2009;13:1409-1416.
  • 9 Njinga NS, Sule MI, Patteh UU, Hassan HS, Usman MA, Haruna MS. Phytochemical and Antidiarrhea Activity of the Methanolic Extract of the Stem Bark of Lannea kerstingii Engl. and K. Krause (Anacardiaceae). J. Nat. Prod. Plant Resour. 2013; 3(3):43-47
  • 10 Koné WM, Kamanzi AK, Traoré D, Betschart B. Anthelmintic activity of medicinal plants used in Northern Côte d'Ivoire against intestinal helminthiasis. Pharm. Biol. 2005;43:72-78.
  • 11 Brain KR, Turner TD. The practical Evaluation of Phytopharmaceutical. 1975;Wright Scientechnica, Bristol.
  • 12 Sofowora A. Medicinal plants and traditional medicine in Africa. 2nd Edn. 1993; Spectrum books limited, Ibadan.
  • 13 Lino A, Deogracios O. The in-vitro antibacterial activity of Annona senegalensis, Securidacca longipendiculata and Steanotaenia araliacea- Ugandan Medicinl plants. Afri. Health Sci. 2006;6(1):31-35
  • 14 Vollekova AD, Sochorova R. Isoquinoline Alkaloids from Mahonia aquifolium stem bark is active against Malassezia Sp. Folia. Microbiologica. 2001;46:107-111.
  • 15 Usman H, Abdulrahman FI, Ladan AH. Phytochemical and Antimicrobial Evaluation of Tribulus terrestris L. (Zygophylaceae) Growing in Nigeria. Res. J Bio. Sci. 2007;2(3):244-247.
  • 16 Obi VI, Onuoha C. Extraction and characterization method of plants and plant products. In: Biological and Agricultural Techniques. Ogbulie, J. L, and O. J. Ojiako (eds). 2000; Webs media publications, Owerri.
  • 17 Hang T, Jin JB, Cho S, Cyang JC. Evaluation of anti-inflammatory effects of baicalein on dextran sulfate sodium-induced colitis in mice. Planta Medica. 2002;68(2):268-271.
  • 18 Vessal M, Hemmati M, Vasei M. Antidiabetic effects of quercetin in streptozocin induced diabetic rats. Comp. Biochem. Physiol. 2003;135:357364.
  • 19 Okuda T, Yoshida T, Hatano T. Ellagitannins as active constituents of medicinal plants. Planta Medica. 1989;55:117-122
  • 20 Adegoke SA, Agada F D, Ogundipe LO. Antibacterial activity of methanol and ethanol leaf extracts of Antidesma venosum and Lannea barteri. African Journal of Microbiology Researc. 7(27), 2013,3442-3447
  • 21 Figueiredo AC, Barroso JG, Pedro LG, Scheffe JJC. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour Frag. J.. 2008;23(4): 213–226
  • 22 Sandhu DS, Heinrich M. The use of health foods, spices and other botanicals in the Sikh community in London. Phytother. Res. 2005;19:633-642.
  • 23 M.P. Guptaa,b,*, P.N. Sol´isa, A.I. Calderon´ a, F. Guinneau-Sinclairc, M. Correab,d, C. Galdamesb, C. Guerraa, A. Espinosaa, G.I. Alvendae, G. Roblese, R. Ocampoe. Medical ethnobotany of the Tribes of Bocas del Toro, Panama. J. Ethnopharmacol. 2005;96:389-401.
  • 24 Shorr AF. Epidemiology and economic impact of meticillin-resistant staphylococcus aureus: review and analysis of the literature. PharmacoEconomics, 2007;25:751-68.
  • 25 Marquart ME, Caballero AR, Chomnawang M, Thibodeaux BA, Twining SS, Callaghan RJ. Identification of a novel secreted protease from Pseudomonas aeruginosa that causes corneal erosions. Invest. Ophthalmol. Vis.. 2005;46:3761-8.