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CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2013; 03(03): 109-112
DOI: 10.1055/s-0040-1703690
Short Communications

PCR: A FAST DIAGNOSTIC INVESTIGATION FOR SPINAL TUBERCULOSIS

M. Ajith Kumar
1   Tejasvini Hospital & SSIOT, Department of Microbiology K. S. Hegde Medical Academy Deralakatte, Nitte University Mangalore - 575 018, Karnataka, India
,
Kini Abhishek R.
2   Tejasvini Hospital & SSIOT, Department of Microbiology K. S. Hegde Medical Academy Deralakatte, Nitte University Mangalore - 575 018, Karnataka, India
,
Shetty Veena A.
3   Associate Professor, Department of Microbiology K. S. Hegde Medical Academy Deralakatte, Nitte University Mangalore - 575 018, Karnataka, India
,
Shantharam Shetty M.
4   Pro-Chancellor, Nitte University, Mangalore - 575 018, Karnataka, India
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Abstract

PCR, as a new faster diagnostic modality for tuberculosis is slowly picking up in our country where nearly six million cases of tuberculosis exists, of which 3% are skeletal tuberculosis. Confirmation of diagnosis by traditional methods takes around couple of weeks and requires a high bacteriological load in the sample.

We are presenting a spinal tuberculosis case where preoperative sample from the surgical empyema proved sufficient for definitive diagnosis of tuberculosis using PCR against hsp65 gene with nucleotide sequence specific for Mycobacterium tuberculosis.

With this case report we have been able to bring about the advantages of PCR i.e. high specificity, early diagnosis and the ease of operations in PCR as compared to other traditional diagnostic modalities for tuberculosis.

Keywords

Mycobacterium tuberculosis - hsp65 gene


Publikationsverlauf

Artikel online veröffentlicht:
26. April 2020

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  • References

  • 1 Tuli SM. Tuberculosis of the Skeletal System. Jaypee brothers. 2004; 3:3-4.
  • 2 Jain AK, Jena SK, Singh MP, Dhammi IK, Ramachadran VG, Dev G. Evaluation of clinico-radiological, bacteriological, serological, molecular and histological diagnosis of osteoarticular tuberculosis. Indian J Orthop 2008;42:173-7.
  • 3 Sareen A, Khare GN, Nath G, Singh S. Role of polymerase chain reaction in osteoarticular tuberculosis. Indian J Orthop 2006;40:264-6.
  • 4 Honore-Bouakline S, Vincensini JP, Giacuzzo V, Lagrange PH, Herrmann JL. Rapid Diagnosis of Extrapulmonary Tuberculosis by PCR: Impact of Sample Preparation and DNA Extraction. J. Clin. Microbiol. 2003;41:2323-2329.
  • 5 Tuli SM. General principles of osteoarticular tuberculosis. Clin. Orthop. 2002;398:11–19.
  • 6 Kim Hyun-Ju, Mun Ho-Suk, Kim Hong, Oh Eun-Ju, Ha Youngju, Bai Gill- Han, et al. Differentiation of Mycobacterial Species by hsp65 Duplex PCR Followed by Duplex-PCR-Based Restriction Analysis and Direct Sequencing: J. Clin. Microbiol. 2006; 44:3855-3862.
  • 7 Folgueira L, Delgado R, Palenque E, Aguado JM, Noriega AR: Rapid diagnosis of Mycobacterium tuberculosis bacteremia by PCR. J. Clin. Microbiol. 1996; 34:512-515.