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CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2013; 03(03): 083-086
DOI: 10.1055/s-0040-1703684
Original Article

IN VIVO ANALYSIS OF LIPID PEROXIDATION AND TOTAL ANTIOXIDANT STATUS IN SUBJECTS TREATED WITH STAINLESS STEEL ORTHODONTIC APPLIANCES

Dilip Daniel Quadras
1   Reader, Department of Orthodontics & Dentofacial Orthopedics, K.V.G Dental College, Sullia 574 327, D.K District, Karnataka
,
U.S. Krishna Nayak
2   Professor & Head of Department, Dean, Department of Orthodontics and Dentofacial Orthopedics, A. B. Shetty Memorial Institute of Dental Sciences, Nitte University, Mangalore 575 018
,
Suchetha Kumari N.
3   Professor, Department of Biochemistry, K. S.Hegde Medical Academy, Deralakatte
,
Prashant Pujari
4   Reader, Department of Orthodontics and Dentofacial Orthopedics Pacific Dental college, Udaipur
,
Vijay R.
5   Central Research Laboratory, A. B. Shetty Memorial Institute of Dental Sciences, Nitte University, Mangalore 575 018
,
Ajay Dethose
6   Central Research Laboratory, A. B. Shetty Memorial Institute of Dental Sciences, Nitte University, Mangalore 575 018
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Abstract

Introduction: Nickel is a strong immunologic sensitizer, although nickel sensitivity has been reported to be lower in subjects who have received orthodontic treatment; perhaps they develop immunological tolerance over the long period of treatment. Hence the present aim of the work was to determine the levels of lipid peroxidation and total antioxidant status in Serum and Saliva in subjects treated with stainless steel Orthodontic appliances.

Methods and Materials: The study group included 25 participants and Samples were taken in different time intervals: Time interval 1- Collection of samples before the appliances fixed, Time interval 2- Collection of sample after one week of fixed appliances, Time interval 3- Collection of sample after three months of fixed appliances each from the Orthodontic Department of A. B.Shetty Memorial institute of dental science.

Lipid peroxidation (Malondialdehyde) level is estimated using Thiobarbituric acid (TBA) method. Total antioxidant level was estimated using Phosphomolybdenum method.

Conclusion: Total antioxidant and Malondialdehyde levels are increased in case of Females compared to Males in serum whereas in case of Saliva Total antioxidant level is increased in Time interval 2 and 3 in Males compared to Females.

Keywords

stainless steel Orthodontic appliances - Lipid peroxidation - Total antioxidant


Publication History

Article published online:
26 April 2020

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  • References

  • 1 Vreeburg KJJ, von Blomberg M, Scheper RJ. Induction of immunological tolerance by oral administration of nickel and chromium. J Dent Res 1984; 63:124-8.
  • 2 Van Hoogstraten IMW, Andersen KE, von Blomberg BME, Boden D, Bruynzeel DP, Burrows D. Reduced frequency of nickel allergy upon oral nickel contact at an early age. Clin Exp Immunol 1991; 85:441-5.
  • 3 Bencko V. Nickel: a review of its occupational and environmental toxicology. J Hyg Epidemiol Microbiol Immunol 1983; 27:237-17.
  • 4 Burrows D. Hypersensivity to mercury, nickel and chromium in relation to dental materials. Int Dent J 1986; 36:30-4.
  • 5 Fisher JR, Rosenblum GA, Thomson BD. Asthma induced by nickel. J Am Med Assoc 1982; 248:1065-6.
  • 6 Hartwing A. Current aspects in metal genotoxicity. Biometals 1995; 8:3-11.
  • 7 Fracasso ME, Perbellini L, Solda S, Talamini G, Franceschetti P. Lead induced DNA strand breaks in lymphocytes of exposed workers: role of reactive oxygen species and protein kinase C. Mutat Res 2002;515:159-69.
  • 8 Mataix J, Quiles JL, Heurtas JR, Battino M, Manas M. Free Radical Biol Med 1998; 24:511-521.
  • 9 Srikrishna R, Suresh DR. Variation of Total antioxidant Status after Ischemic stroke, British J Med Practision 2009; 2(1):35-37.
  • 10 Bast, Haenen CD. Reactive oxygen species and airway inflammation. Free Radio Bio Med 2003; 91(3):2-13.