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CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2013; 03(03): 038-041
DOI: 10.1055/s-0040-1703675
Original Article

SMOKING GENOTOXICITY IN PERIPHERAL BLOOD LYMPHOCYTES USING THE COMET ASSAY

Shobha S. Shetty
1   Associate Professor, Department of General Medicine, K. S. Hegde Medical Academy, Deralakatte, Nitte University, Mangalore 575 018, Karnataka, India
,
Hrishikesh Nachane
2   Intern, K. S. Hegde Medical Academy, Deralakatte, Nitte University, Mangalore 575 018, Karnataka, India
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Abstract

Background: Smoking has been shown to have a positive effect on DNA damage in almost all the cells of the body. Quantitative analysis of this damage will help in assessing the etiopathogenesis of various nicotine induced damage to the body. Comet assay has been an emerging tool in this regard and hence was applied by us to estimate the severity of DNA damage in smokers.

Aims & Objectives: To evaluate the DNA genotoxicity in peripheral blood lymphocytes in smokers and their comparison with non smokers & assess the quantitative damage.

Materials and methods: 30 smokers & 20 non smokers were recruited & their peripheral blood was taken for the comet assay to look for Olive moment & Tail moment to quantitatively assess the DNA damage due to cigarette smoking.

Results: In our study there was no significant difference in the analysis of DNA damage (with regard to tail moment & olive moment) in smokers versus non smokers (P value: more than 0.05).

Conclusions: Though smoking is known to cause DNA damage, we did not find significant differences between the two groups probably due to other multifactorial etiologies for genotoxicity.

Keywords

Comet Assay - DNA Damage - Genotoxicity - Smoking


Publikationsverlauf

Artikel online veröffentlicht:
27. Juni 2020

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Thieme Medical and Scientific Publishers Private Ltd.
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  • References

  • 1 Gurel E, Orta T, Gunebakan S, Utkusavao A, Kolusayin Ozar MO. Can micronucleus technique predict the risk of lung cancer in smokers? Tuberk Toraks 2005;53:225-30.
  • 2 Wiesman H, Halliwell B. Damage to DNA by reactive oxygen species and nitrogen species: role of inflammatory disease and progression to cancer. Biochem J 1996;313:17-29.
  • 3 Halliwell B. Oxidants and human disease: some new concepts. FASEB J 1987;1:358-364.
  • 4 Nakayama T, Kaneko M, Kodama M, Nagata C. Cigarette smoke induces DNA single-strand breaks in human cells. Nature 1985;314:462–4.
  • 5 Yang Q, Hergenhahn M, Weninger A, Bartsch H. Cigarette smoke induces direct DNA damage in the human B-lymphoid cell line. Carcinogenesis 1999;20:1769–75.
  • 6 Kato T, Nagasawa H, Warner C, Okayasu R, Bedford JS. Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wildtype CHO cells. Int J Radiat Biol 2007;83:583–91.
  • 7 Albino AP, Huang X, Jorgensen ED, Gietl D, Traganos F, Darzynkiewicz Z. Induction of DNA double-strand breaks in A549 and normal human pulmonary epithelial cells by cigarette smoke is mediated by free radicals. Int J Oncol 2006;28:1491–1505.
  • 8 Kassie F, Parzefall W, Knasmuller S. Single cell gel electrophoresis assay: a new technique for human biomonitoring studies. Mutat Res 2000;463:13–31.
  • 9 Rojas E, Lopez MC, Valverde M. Single cell gel electrophoresis assay: methodology and applications. J Chromatogr B Biomed Sci Appl 1999;722:225–254.
  • 10 Calini V, Urani C, Camatini M. Comet assay evaluation of DNA singleand double-strand breaks induction and repair in C3H10T1/2 cells. Cell Biol Toxicol 2002;18:369–379.
  • 11 Chu G. Double strand break repair. J Biol Chem 1997;272:24097–100.
  • 12 Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H et al., Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen 2000;35:206–21.
  • 13 Hartmann A, Agurell E, Beevers C. et al. Recommendations for conducting the in vivo alkaline comet assay. Mutagenesis 2003;18:45–51.
  • 14 Brendler-Schwaab S, Hartmann A, Pfuhler S, Speit G. The in vivo comet assay: use and status in genotoxicity testing. Mutagenesis 2005;20:245–54.
  • 15 Moller P, Knudsen LE, Loft S, Wallin H. The comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors. Cancer Epidemiol 2000; Biomarkers Prev;9:1005–15.
  • 16 Faust F, Kassie F, Knasmuller S, Boedecker RH, Mann M, Mersch-Sundermann V. The use of the alkaline comet assay with lymphocytes in human biomonitoring studies. Mutat Res 2004;566:209–29.
  • 17 Hellman B, Vaghef H, Bostro¨m B. The concepts of tail moment and tail inertia in the single cell gel electrophoresis assay. Mutat Res 1995; 336:123-31.
  • 18 Manuel F. Ellahueñe, Luz Patricia Pérez-Alzola, Mauricio Farfán-Urzua, et al. Cancer Epidemiol Biomarkers Prev 2004;13:1223-9.
  • 19 Sram RJ, Binkova B. Molecular epidemiology studies on occupational and environmental exposure to mutagens and carcinogens, 1997–1999. Environ Health Perspect 2000;108:57–70.
  • 20 Tates AD, van Dam FJ, de Zwart FA, et al. Biological effect monitoring in industrial workers from the Czech Republic exposed to low levels of butadiene. Toxicology 1996;113, 91–99.
  • 21 Ayse G Zamani, H Gul Durakbasi-Dursun, Sennur Demirel, Aynur Acar, Evaluation of smoking genotoxicity in Turkish young adults, Indian J Hum Genet 2011;17:7-12
  • 22 Polasa K, Naidu AN, Ravindranath I, Krishnaswamy K. Inhibition of B[a]P induced strand breaks in presence of curcumin. Mutat Res 2004;557:203–13.