Ferrum phosphoricum D12 Treatment Affects J774A.1 and 3T3-L1 Cells Proliferation and Gene Expression of Inflammation, Oxidative Stress and Iron Metabolism-Related Proteins
05 February 2020 (online)
Ferrum phosphoricum (FP), the so-called “cell salt”, is prescribed as a homeopathic remedy to treat the early stage of fever and inflammation in cases of cold or flu, muscle fatigue and anemia. We aimed to analyse the molecular mechanisms of action of FP D12 tablet solution in vitro, on cell proliferation and gene expression of inflammation, oxidative stress and iron metabolism-related proteins in mouse J774A.1 macrophages and 3T3-L1 pre-adipocytes.
Cell proliferation was examined using the MTT test. RT qPCR analyses followed by the 2−ΔΔCt calculation method were performed to estimate gene expression changes. Statistical analyses were done by GraphPad Prism V6 software; p < 0.05 was considered as significant. FP effects were compared to placebo treatment (PT) and to untreated cells.
FP significantly stimulated proliferation of J774A.1 and 3T3-L1 cells, by 11% and 15% respectively, in contrast to PT in the respective concentrations.
FP vs. PT significantly induced gene expression of Ferritin FTH1 and Beta-2-Microglobulin proteins (by 8-fold and 2.5-fold respectively) and IREB2 transcription factor (by 4-fold), and induced a slight decrease in myosin 1E (by 0.4-fold) gene expression levels in macrophages; whereas in pre-adipocytes FTH1 (by 3-fold) and IREB2 (by 15-fold) gene expression was induced. Significant stimulation of antioxidant enzymes GPX-1 (by 1.2-fold) in macrophages and GCL (by 11-fold) in pre-adipocytes by FP was observed. Significant induction in the gene expression of IL-1β (by 3.5-fold) in macrophages and of IL-6 (by 20-fold), TNFα (by 16-fold) and NOXO1 (by 17-fold) in pre-adipocytes was measured.
Results indicate that FP in D12 potency may exhibit immunostimulatory, antioxidant and increased iron uptake potential, possibly by inducing changes in gene expression levels.
Keywords: Ferrum phosphoricum, proliferation, gene expression, macrophages, pre-adipocytes