Subscribe to RSS
Download PDF
DOI: 10.1055/s-0039-3402085
Evaluation of Hydroalcoholic Extract of Convolvulus pluriculis (Shankapushpi) for Standardization by Colorimetric Method: A Preliminary Report
Abstract
Aim This study was aimed to evaluate the hydroalcoholic extract of Convolvulus pluricaulis (HACP) for standardization using colorimetric method.
Materials and Methods Establishment of standardization for the Ayurvedic formulations is most important for its chemical compounds, biological action, and its quality reassurance in production and manufacturing of traditional herbal medicines. As most of the drugs are standardized, drug companies are using substitute drugs instead of true drugs. So to make finest superiority drugs, it is necessary to validate the raw drugs. Observing the existing trend in mind, HACP was subjected to standardize procedures for the phytochemical tests. The separation of the bioactive substances from the HACP was performed using both manual methods and high-profile thin-layer chromatography (HPTLC).
Results From this study, it is revealed that the seed contains alkaloids, carbohydrates, steroids, tannins, terpenoid, and phenol which gave the medicine numerous therapeutic properties.
Conclusion The study was rapid, reproducible, and could be used for routine monitoring of various biological properties of HACP.
Publication History
Received: 26 September 2019
Accepted: 21 November 2019
Article published online:
05 February 2020
© .
Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India
-
References
- 1 Verma S, Sinha R, Singh V, Tanwar S, Godara M. Antibacterial activity of methanolic extract of whole plant of Convolvulus pluricaulis choisy. J Pharm Res 2011; 4 (12) 4450-4452
- 2 Verma S, Singh V, Tanwar S. Pharmacognostic validation of whole plant of Convolvulus pluricaulis Choisy (Convolvulaceae). Int J Pharm Pharm Sci 2012; 4 (01) 241-246
- 3 Bhowmik D, Kumar KS, Paswan S, Srivatava S, Yadav A, Dutta A. Traditional Indian herbs Convolvulus pluricaulis and its medicinal importance. J Pharmacogn Phytochem 2012; 1 (01) 44-51
- 4 Singh GK, Bhandari A. Textbook of Pharmacognosy. 1st ed. New Delhi, India: CBS Publishers; 2000: 193-194
- 5 Shalavadi MH. Physicochemical and phytochemical screening of Convolvulus pluricaulis collected from Bagalkot, Karnataka. IJGP 2018; 12 (03) S625-S632
- 6 Bharkatiya M, Goyal A, Gupta GD, Gaud RS. Memory enhancers. Indian Pharmacist 2006; 5 (08) 32-36
- 7 Nordberg A, Svensson AL. Cholinesterase inhibitors in the treatment of Alzheimer's disease: a comparison of tolerability and pharmacology. Drug Saf 1998; 19 (06) 465-480
- 8 Rakhit S, Basu NK. Investigations on Convulvulus pluricaulis Chois. Part II. Indian J Pharm 1958; 20: 357-360
- 9 Champatisingh D, Sahu PK, Pal A, Nanda GS. Anticataleptic and antiepileptic activity of ethanolic extract of leaves of Mucuna pruriens: A study on role of dopaminergic system in epilepsy in albino rats. Indian J Pharmacol 2011; 43 (02) 197-199
- 10 WHO, Quality Control Methods for Medicinal Plant Materials. Geneva, Switzerland: World Health Organization; 1998: 65-67
- 11 Government of India. The Ayurvedic Pharmacopeia of India. Part I. 1st ed. Vol. 1. New Delhi, India: Department of AYUSH, Ministry of Health and Family Welfare; 2001: 214
- 12 Government of India. Indian Pharmacopoeia. 1st ed. Vol. 1. New Delhi, India: Ministry of Health and Family Welfare; 2010: 10-146
- 13 Nayeem AA, Khatun A, Rahman MS, Rahman M. Evaluation of phytochemical and pharmacological properties of Mikania cordata (Asteraceae) leaves. J. Pharmacognosy Phytother 2011; 3 (08) 118-123
- 14 Evans WC. Trease and Evans Pharmacognosy. 14th ed. Hartcourt Brace and Company. Asia Pvt. Ltd; Singapore: 1997: 226-227
- 15 Wagner H. Pharmazeutische Biology. 5th ed. Stuttgart, Germany: Gustav fisher Verlag; 1993
- 16 Neelima N, Devidas NG, Sudhakar M, Kiran VJ. A preliminary phytochemical investigation on the leaves of Solanum xanthocarpum. Int J Res Ayurveda Pharm 2011; 2 (03) 845-850
- 17 Foulger JH. The use of the Molisch (d-naphthol) reactions in the study of sugars in biological fluids. J Biol Chem 1931; 92: 345-353
- 18 Fehling H. Die quantitative Bestimmung von Zucker und Stärkmehl mittelst Kupfervitriol. [The quantitative determination of sugar and starch by means of copper sulfate]. Annalen der Chemie und Pharmacie 1849; 72 (01) 106-113
- 19 Simoni RD, Hill RL, Vaughan M. Benedict's solution, a reagent for measuring reducing sugars: the clinical chemistry of stanley R. Benedict. J. Biol. Chem 2002; 277 (16) 10-11
- 20 Nath MC, Chakravorty MK, Chowdhury SR. Liebermann-Burchard reaction for steroids. Nature 1946; 157 (3978) 103
- 21 Levine VE, Richman E. A test for cholesterol based upon the use of sulphuric acid containing selenious acid. Proc. Soc. Exper. Biol. A. Med. 1930; 27 (08) 832-833
- 22 Auwal MS, Saka S, Mairiga IA, Sanda KA, Shuaibu A, Ibrahim A. Preliminary phytochemical and elemental analysis of aqueous and fractionated pod extracts of Acacia nilotica (Thorn mimosa) Vet Res Forum. 2014; 5 (02) 95-100
- 23 Zohra SF, Meriem B, Samira S, Muneer MA. Phytochemical screening and identification of some compounds from mallow. J Nat Prod Plant Resour 2012; 2 (04) 512-516
- 24 Usman H, Abdulrahman F, Usman A. Qualitative phytochemical screening and in vitro antimicrobial effects of methanol stem bark extract of Ficus thonningii (Moraceae) Afr J Tradit Complement Altern Med. 2009; 6 (03) 289-295
- 25 Pasto DJ, Johnson CR, Miller M. Experiments and Techniques in Organic Chemistry. NJ. Englewood Cliffs 1992; 00
- 26 Nidhin PS, Yaligar MG, Arun Raj GR, Koppala Narayana Sunil Kumar, Ravi M. Standardization of Harithaki (Terminalia chebula Retz.) and Trivrit (Operculina turpethum L.) churna: Two important drugs used for purgation in Ayurveda. J Pharmacogn Phytochem 2015; 4 (01) 203-209
- 27 Mosettig E. The synthesis of aldehydes from carboxylic acids. Org React. 2004; 8: 218-257
- 28 Ruskin RS, Kumari BV, Chitarasu T. Qualitative phytochemical and FTIR analysis of root extracts of Canthium parviflorum Lam. J Chem Pharm Sci 2014; 2: 122-127
- 29 Geiss F. Fundamentals of Thin Layer Chromatography Planar Chromatography. Huthig: Heidelberg 1987; 854-857
- 30 Indian Pharmacopoeia Committee. Pharmacopoeia of India, India. 3rd ed. Ministry of Health & Family Welfare. Controller of Publications; 1985
- 31 World Health Organization. The International Phamacopoeia. 3rd ed. Geneva, Switzerland: World Health Organization; 1994
- 32 Agarwa P, Sharma B, Fatima A, Jain SK. An update on Ayurvedic herb Convolvulus pluricaulis Choisy. Asian Pac J Trop Biomed 2014; 4 (03) 245-252
- 33 Chandel U, Kharoliwal S. Phytochemical estimation of convolvulus pluricaulis. World Journal Of Pharmacy And Pharmaceutical Sciences Choisy 2017; 6 (10) 1074-1082
- 34 Prasad SB, Sharma A. Standardisation of Convolvulus pluricaulis Choisy Herbs collected from Jalandhar, Punjab. IJPPR 2016; 8 (08) 1412-1416