Download PDF
CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2019; 09(03): 116-120
DOI: 10.1055/s-0039-3402084
Original Article

Amelioration of Diabetic Nephropathy in Streptozotocin-Induced Diabetic Rats by Acacia catechu Leaves Extract

Prima D’souza
1   Department of Anatomy, K. S. Hegde Medical Academy, Deralakatte, Mangalore, Karnataka, India
,
Rajendra Holla
2   Department of Pharmacology, K. S. Hegde Medical Academy, Deralakatte, Mangalore, Karnataka, India
,
Gangadhara Swamy
3   Department of Anatomy, Subbaiah Institute of Medical Sciences and Research Center, Shivamogga, Karnataka, India
› Author Affiliations
› Further Information
   Permissions and Reprints

Abstract

Objective The present study was performed to evaluate the ethanolic extract of leaves of Acacia catechu (A. catechu) for its effect on streptozotocin (STZ)-induced diabetes mellitus (DM) and its renal complications in male Wistar albino rats.

Materials and Methods Male Wistar albino rats were grouped into control (A), STZ-induced DM (B), STZ-induced DM rats with A. catechu orally of 75 mg/kg body weight (kbw) for 35 days (C), with each group having six rats (n = 6) weighing between 200 to 250 g each. Group A receives only water, orally; group B receives a single dose of STZ at 45 mg/kbw intraperitoneal administration (IP); group C receives STZ IP and oral A. catechu for 35 days. On the 36th day, animals were euthanized, the kidney tissues were analyzed for biochemical parameters, such as GOT (glutamic oxaloacetic transaminase), GPT (glutamic pyruvic transaminase), oxidative stress assessment parameters, and histopathological studies.

Results In group C rats, activities of the enzymes were nearer to group A when compared with group B. Histopathological findings were also suggesting that renal toxicity were observed at a lesser extent in group C.

Conclusion The ethanolic extract of A. catechu signified as nephroprotective effect. The present data could provide adequate confirmation of the efficacy of ethanolic extract of leaves of A. catechu for further experimental studies on a standardized formulation.

Keywords

diabetes mellitus - Streptozotocin - Acacia catechu - nephroprotective


Publication History

Received: 26 September 2019

Accepted: 18 November 2019

Article published online:
05 February 2020

© .

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

  • References

  • 1 Whiting DR, Guariguata L, Weil C, Shaw J. IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030. Diabetes Res Clin Pract 2011; 94 (03) 311-321
    CrossrefPubMedGoogle Scholar
  • 2 Ritz E, Orth SR. Nephropathy in patients with type 2 diabetes mellitus. N Engl J Med 1999; 341 (15) 1127-1133
    CrossrefPubMedGoogle Scholar
  • 3 Matough FA, Budin SB, Hamid ZA, Alwahaibi N, Mohamed J. The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos Univ Med J 2012; 12 (01) 5-18
    CrossrefPubMedGoogle Scholar
  • 4 Je HD, Shin CY, Park HS, Huh IH, Sohn UD. The comparison of vitamin C and vitamin E on the protein oxidation of diabetic rats. J Auton Pharmacol 2001; 21 (5-6) 231-236
    CrossrefPubMedGoogle Scholar
  • 5 Yildirim O, Büyükbingöl Z. In vivo effect of vitamin C with cobalt on oxidative stress in experimental diabetic rat kidney. Diabetes Nutr Metab 2003; 16 (04) 208-213
    PubMedGoogle Scholar
  • 6 Savu O, Ionescu-Tirgoviste C, Atanasiu V, Gaman L, Papacocea R, Stoian I. Increase in total antioxidant capacity of plasma despite high levels of oxidative stress in uncomplicated type 2 diabetes mellitus. J Int Med Res 2012; 40 (02) 709-716
    Google Scholar
  • 7 Viswanathan V. Prevention of Diabetic Nephropathy: A diabetologist’s perspective. Indian J Nephrol 2004; 14: 157-162
    Google Scholar
  • 8 Rosario RF, Prabhakar S. Lipids and diabetic nephropathy. Curr Diab Rep 2006; 6 (06) 455-462
    CrossrefPubMedGoogle Scholar
  • 9 Venkatesh S, Reddy GD, Reddy BM, Ramesh M, Rao AV. Antihyperglycemic activity of Caralluma attenuata. Fitoterapia 2003; 74 (03) 274-279
    CrossrefPubMedGoogle Scholar
  • 10 Deeds MC, Anderson JM, Armstrong AS. et al. Single dose streptozotocin-induced diabetes: considerations for study design in islet transplantation models. Lab Anim 2011; 45 (03) 131-140
    CrossrefPubMedGoogle Scholar
  • 11 GU D, Arnush M, Sarvetnic N. Endocrine/exocrine intermediate cells in STZ treated Ins-IFNgamma transgenic mice. Pancreas 1997; 15 (03) 246-250
    CrossrefPubMedGoogle Scholar
  • 12 Tesch GH, Allen TJ. Rodent models of streptozotocin-induced diabetic nephropathy. Nephrology (Carlton) 2007; 12 (03) 261-266
    CrossrefPubMedGoogle Scholar
  • 13 Lakshmi T, Anitha R, Geetha RV. Acacia catechu willd - A gift from ayurveda to mankind - A Review. T. Ph. Res. 2011; 5 (02) 273-293
    PubMedGoogle Scholar
  • 14 Monu MP, Kadian R, Sharma K. Phytopharmacology of acacia catechu willd: a review. World J Pharm Sci 2014; 3 (11) 1380-1389
    Google Scholar
  • 15 Pfaller W, Gstraunthaler G. Nephrotoxicity testing in vitro-what we know and what we need to know. Environ Health Perspect 1998; 106 (02) 559-569
    PubMedGoogle Scholar
  • 16 Nadumane KV, Nair S. Evaluation of the anticancer and cytotoxic potentials of Acacia catechu extracts in vitro. J Nat Pharmaceuticals 2011; 2 (04) 190-195
    Google Scholar
  • 17 Ray D, Sharatchandra K, Thokchom I. Antipyretic, antidiarrhoeal, hypoglycaemic and hepatoprotective activities of ethyl acetate extract of Acacia catechu Wild in albino rats. Ind J Pharmacol 2006; 38 (06) 408-413
    CrossrefGoogle Scholar
  • 18 Gayathri DV, Lanitha J, Devi R, Sreekala. Prabhakaran VA. Pharmacognostical studies on Acacia catechu willd and identification of antioxidant principles. Int J Pharm Sci 2011; 3 (02) 108-111
    Google Scholar
  • 19 Guleria S, Tiku AK, Singh G, Vyas D, Bhardwaj A. Antioxidant activity and protective effect against plasmid DNA strand scission of leaf, bark, and heartwood extracts from Acacia catechu. J Food Sci 2011; 76 (07) C959-C964
    CrossrefPubMedGoogle Scholar
  • 20 Pingale SS. Heptoprotection by Acacia catechu in CCL4 Induced Liver Dysfunction. Inter J of Pharmaceutical Sciences Rev. and Res 2010; 5 (01) 150-154
    Google Scholar
  • 21 Nagarchi K, Ahmed S, Sabus A, Saheb SH. Effect of STZ on Glucose levels in Albino Wister Rats J. Pharm. Sci. & Res. 2015; 7 (02) 67-69
    PubMedGoogle Scholar
  • 22 Mohun A, Cook P. Simple methods for measuring serum levels of the glutamate-oxaloacetate and glutamic pyruvic transaminases in routine laboratory. J Clin Puth 1957; 10: 394-399
    Google Scholar
  • 23 Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971; 44 (01) 276-287
    CrossrefPubMedGoogle Scholar
  • 24 Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959; 82 (01) 70-77
    CrossrefPubMedGoogle Scholar
  • 25 Teoh SL, Abd Latiff A, Das S. Histological changes in the kidneys of experimental diabetic rats fed with Momordica charantia (bitter gourd) extract. Rom J Morphol Embryol 2010; 51 (01) 91-95
    PubMedGoogle Scholar
  • 26 Haneda M. [Mechanisms for the development and progression of diabetic nephropathy]. Nihon Rinsho 2006; 64 (Suppl. 02) 427-432
    PubMedGoogle Scholar
  • 27 Sharma S, Kulkarni SK, Chopra K. Curcumin, the active principle of turmeric (Curcuma longa), ameliorates diabetic nephropathy in rats. Clin Exp Pharmacol Physiol 2006; 33 (10) 940-945
    CrossrefPubMedGoogle Scholar
  • 28 Mansouri E, Panahi M, Ghaffari MA, Ghorbani A. Effects of grape seed proanthocyanidin extract on oxidative stress induced by diabetes in rat kidney. Iran Biomed J 2011; 15 (03) 100-106
    PubMedGoogle Scholar
  • 29 Uzel N, Sivas A, Uysal M, Oz H. Erythrocyte lipid peroxidation and glutathione peroxidase activities in patients with diabetes mellitus. Horm Metab Res 1987; 19 (02) 89-90
    Article in Thieme ConnectPubMedGoogle Scholar
  • 30 Pasaoglu H, Sancak B, Bukan N. Lipid peroxidation and resistance to oxidation in patients with type 2 diabetes mellitus. Tohoku J Exp Med 2004; 203 (03) 211-218
    CrossrefPubMedGoogle Scholar
  • 31 Sandhya Pillai Nair NC. Shah, R.M Shah. Alteration in enzymatic antioxidant defense in diabetes mellitus. Biomed Res 2012; 23 (03) 402-404
    Google Scholar
  • 32 Baghshani H, Ghodsi V. Evaluation of some enzymatic changes in the liver and kidney of rats following exposure to sub-lethal concentration of potassium cyanide. Iranian Journal of Toxicology 2016; 10 (04) 9-12
    Google Scholar
  • 33 Zafar M. et al. Altered kidney morphology and enzymes in STZ induced diabetic rats. Int J Morphol 2009; 27 (03) 783-790
    Google Scholar
  • 34 Saha MR, Dey P, Begum S. et al. Effect of Acacia catechu (L.f.) Willd. on oxidative stress with possible implications in Alleviating Selected Cognitive Disorders. PLoS One 2016; 11 (03) e0150574
    CrossrefPubMedGoogle Scholar