Cryodehydration Technique Applied to Anatomical Segments
10. November 2017
17. Oktober 2019
04. Dezember 2019 (online)
Introduction The present study describes a variation of the cryodehydration technique, applied to prepare biological tissues by repetitions of section freezing and thawing (SFT). First developed for muscles and then viscera, the aim of this process as presented here is to obtain anatomical segments to be used in anatomy classes, general exhibitions and museums.
Materials and Methods The animal, previously fixed in 10% formalin, must be frozen and then sectioned in longitudinal, transverse and horizontal slices of the body-axis. It has two steps: the “burning phase,” in which ice crystal formation and water dilatation cause micro ruptures in the tissue; and the “dehydration phase,” developed to dry out the slices segments slowly and, at the same time, to impregnate tissues with wood glue.
Results The obtained slices present several advantages, such as being dry segments, of light weight, and being rapidly used, easily stored and promptly studied. Also, it is possible to study the different organs of one segment and, by apposition, remount an entire animal, enabling a dynamic study.
Conclusion This very simple and inexpensive technique produces anatomical preparations with high durability and resistance, which are very helpful in practical and theoretical classes.
- 1 Koonz CH, Ramsbotton JM. A method for studying the histological structure of frozen products. I. Poultry. J Food Sci 1939; 4 (02) 117-128
- 2 Hiner RL, Hankins OL. Temperature of freezing affects tenderness of beef. Food Ind 1947; 19: 108-111
- 3 Teixeira A, Teixeira Filho A, Guarenti VPJ. Desidratação de músculos no preparo de peças anatômicas. Rev Brasil Ciências Morfol 1990; 7/8 (1/2): 45-47
- 4 Teixeira Filho A, Guarenti VPJ, Teixeira A, Carambula SF, Cruzati A, Brucker PF. The cryodehydration technique applied to cavitary and parenchymatous viscera. Braz J Morphol Sci 1996; 13 (02) 177-180
- 5 Teixeira Filho A, Busch LC. Ein Dehydratations-Verfahren zur Herstellung von Körperscheiben. Paper presented at: Viertes Treffen der Ostsee-Anatomen; 1994 ; Rostock