Planta Med 2019; 85(18): 1566-1567
DOI: 10.1055/s-0039-3400144
Main Congress Poster
Poster Session 2
© Georg Thieme Verlag KG Stuttgart · New York

Phytochemical characterization and in vitro assessment of oral-health related pharmacological activities of Salvadora persica leaves

S Kobetitsch
1   Institute of Pharmaceutical Sciences, University of Graz,, Universitätsplatz 4, 8010 Graz, Austria
,
B Michalak
2   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw,, Banacha 1, 02-097 Warsaw, Poland
,
O Kunert
1   Institute of Pharmaceutical Sciences, University of Graz,, Universitätsplatz 4, 8010 Graz, Austria
,
A Mazen
3   Department of Botany, Faculty of Science, Sohag University,, Sohag, Egypt
,
N Kretschmer
1   Institute of Pharmaceutical Sciences, University of Graz,, Universitätsplatz 4, 8010 Graz, Austria
,
P Raab
1   Institute of Pharmaceutical Sciences, University of Graz,, Universitätsplatz 4, 8010 Graz, Austria
,
R Bauer
1   Institute of Pharmaceutical Sciences, University of Graz,, Universitätsplatz 4, 8010 Graz, Austria
,
AK Kiss
2   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw,, Banacha 1, 02-097 Warsaw, Poland
,
EM Pferschy-Wenzig
1   Institute of Pharmaceutical Sciences, University of Graz,, Universitätsplatz 4, 8010 Graz, Austria
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

The stems and roots of the ever-green shrub Salvadora persica L. (Salvadoraceae) are important sources of plant-based tooth-cleaning sticks (miswak) commonly used for oral hygiene throughout the Arabian peninsula and the wider Muslim world [1] [2]. Also the leaves of the plant are traditionally eaten as salad and used for medicinal purposes [3], but studied in less detail so far.

Therefore, the aim of this study was the phytochemical and pharmacological investigation of S. persica leaves.

The phytochemical composition of a methanolic S. persica leaf extract was assessed by LC-HRMS analysis. Some of the major compounds were assigned as the glucosinolate glucotropeolin and as a series of kaempferol and isorhamnetin glycosides.

Furthermore, the oral health promoting-effects of the methanolic leaf extract (SPML), of glucotropaeolin (GT) and of benzylisothiocyanate (BT), a glucotropeolin degradation product formed by myrosinase-catalyzed cleavage ([Fig. 1]), were assessed in cell-based assays on anti-inflammatory and anticancer activity.

Only BT was able to inhibit the growth of HNO97 human tongue carcinoma cells. Also LPS/IFN-γ-induced NO production in RAW264.7 cells was only inhibited by BT. However, SPML as well as GT and BT potently inhibited IL-1β, TNF-α and IL-8 production in neutrophils stimulated with LPS. Moreover, SPML and BT strongly inhibited ROS production in this model.

Since primed and hyperactivated neutrophils are known to be involved in inflammatory oral diseases like periodontitis [4], the effects observed for SPML, GT and BT in stimulated neutrophils may substantiate the traditional use of S. persica leaf preparations for oral health purposes.

Zoom Image
Fig. 1 Myrosinase- catalyzed degradation of glucotropaeolin to benzylisothiocyanate.
 
  • References

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  • 2 “Salvadora persica” (PDF). Agroforestry Database 4.0.2009. Retrieved 5 April 2019
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