Subscribe to RSS
DOI: 10.1055/s-0039-3400133
Antimicrobial activity and phytochemical analysis of Combretum collinum leaves extract
Publication History
Publication Date:
20 December 2019 (online)
Herbal medicinal products show significant contributions to mankind in the treatment of infections with pathogenic bacteria and fungi. The present study aimed at finding the antimicrobial potential of ethanolic leaf extracts from C. collinum. The phenolic profile of the ethanolic extract could already be characterized in previous studies [1]. However knowlegde about the lipophilic part of the extract is still scarce. In order to obtain further information on the lipholilic components of C.collinum, a GC-MS analysis of the hexane extract of the leaves was performed.
During the GC-MS-analysis 22 new substances including myristic acid, palmitic acid, stearic acid, oleic acid, lignoceric acid, oleic acid amide, campesterol, stigmasterol and beta-sitosterol could be identified by comparison with spectra from the NIST2 database.
The antibacterial activity of the ethanolic extract of C. collinum against eight bacterial strains was evaluated by comparing the diameters of the inhibition zone with standard antibiotics. Subsequently, the MICs of the extract were tested against selected bacterial strains that had been shown to be sensitive.
Inhibition zones were found for the ethanolic extract of C.collinum for the following microorganisms: S.epidermidis, S.aureus, MRSA, K.pneumoniae, Enterococcus, P.aeruginosa. The effect was strongest against S.epidermidis, S.aureus and MRSA. MIC values resulted in 275.0 µg/ml for S. epidermidis respectively 385.5 µg/ml for MRSA.
The tested ethanolic leaf extract showed a promising antibacterial effect. In further experiments it has to be investigated which volatile components of the hexane extract also occur in the ethanolic extract and thus can contribute to the antibacterial effect.
-
References
- 1 Marquardt P, Seide R, Fester K. Antioxidant capacity and phenolic profiling of Combretum collinum from Benin Zeitschrift für Phytotherapie 2017; 38.S (01) P17