Planta Med 2019; 85(18): 1547
DOI: 10.1055/s-0039-3400087
Main Congress Poster
Poster Session 2
© Georg Thieme Verlag KG Stuttgart · New York

Selected extracts from Potentilla alba modulate the viability of normal and cancerous cells of the human colon epithelium

K Kowalik
1   Department of Virology and Immunology, Maria Curie-Skłodowska University,, ul Akademicka 19, 20-033 Lublin, Poland
,
M Pięt
1   Department of Virology and Immunology, Maria Curie-Skłodowska University,, ul Akademicka 19, 20-033 Lublin, Poland
,
R Paduch
1   Department of Virology and Immunology, Maria Curie-Skłodowska University,, ul Akademicka 19, 20-033 Lublin, Poland
,
A Wiater
2   Department of Industrial Microbiology, Maria Curie-Skłodowska University,, ul. Akademicka 19, 20-033 Lublin, Poland
,
M Pleszczyńska
2   Department of Industrial Microbiology, Maria Curie-Skłodowska University,, ul. Akademicka 19, 20-033 Lublin, Poland
,
M Tomczykowa
3   Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Białystok,, ul. Mickiewicza 2A, 15-222 Białystok, Poland
,
JW Strawa
4   Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Białystok,, ul. Mickiewicza 2A, 15-230 Białystok, Poland
,
S Granica
5   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Warsaw Medical University,, ul. Banacha 1, 02-097 Warsaw, Poland
,
M Tomczyk
4   Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Białystok,, ul. Mickiewicza 2A, 15-230 Białystok, Poland
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

Potentilla alba (Rosaceae) has been used in the traditional folk medicine for several hundred years. However, data on the cytotoxicity effects of this plant are limited [1],[2]. In the study the following extracts from aerial parts of P. alba were used: MeOH (PAL1), 50% MEOH (PAL2), water (PAL3), Et2O (PAL4), EtOAc (PAL5), n-BuOH (PAL6) and 70% acetone (PAL7). MTT and NR assays for assessing cells viability were used. The tests were carried out on human colon tumour (HT29) and normal epithelial (CCD 841 CoTr) cell cultures. In NR uptake method, PAL7 extract (25 µg/mL) had the strongest cytotoxic (by 18% compared to the control) potential against cancer cells. Extracts PAL1, PAL4, PAL5 and PAL6 significantly decreased the viability of cells (to 83% compared to control) at 225 µg/mL concentration. PAL4 extract (125 µg/mL), was the most active against normal cells decreasing their viability to 77% comparing to the control. In MTT method, PAL2, PAL4, PAL7 extracts at concentrations lower than 125 µg/mL increased up to 60% the succinate dehydrogenase activity. When normal cells were analysed, the enzyme activity was not affected by the PAL2 and PAL3 extracts. The remaining extracts in a concentration dependent manner stimulated the enzyme activity in normal cells. P. alba extracts depending on the concentration used, type of solvent and type of cells exerted differential effects in the context of cell viability and the action of selected Krebs cycle enzymes. An analytical approach based on LC-ESI-MS was also applied to obtain phytochemical profile of all extracts.

 

  • References

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