Planta Med 2019; 85(18): 1532
DOI: 10.1055/s-0039-3400043
Main Congress Poster
Poster Session 2
© Georg Thieme Verlag KG Stuttgart · New York

HPLC-based activity profiling of Haplophyllum tuberculatum In vitro activity against Madurella mycetomatis

A Mahmoud
1   Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Socinstrasse 57, 4000 Basel, Switzerland;
2   University of Basel, Petersplatz 1, 4003 Basel, Switzerland;
3   Faculty of Pharmacy, University of Khartoum, Sudan
,
S A Algaffar
4   Faculty of Pharmacy, University of Science and Technology, Omdurman, Sudan
,
P Mäser
1   Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Socinstrasse 57, 4000 Basel, Switzerland;
2   University of Basel, Petersplatz 1, 4003 Basel, Switzerland;
,
S Khalid
3   Faculty of Pharmacy, University of Khartoum, Sudan
4   Faculty of Pharmacy, University of Science and Technology, Omdurman, Sudan
,
M Hamburger
5   Department of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, 4056 Basel, Switzerland
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

Eumycetoma is a chronic debilitating inflammatory fungal infection caused mainly by Madurella mycetomatis. Eumycetoma is endemic in most tropical and subtropical countries and was recently enlisted by the WHO among the neglected tropical diseases (NTDs) [1]. Current treatment includes long-term treatment with itraconazole and surgical intervention in most of the cases. Given limited efficacy, frequent side effects, and high cost of treatment, there is an urgent need for new antimycetomal drugs.

A subset of extracts from a repository of Sudanese medicinal plants traditionally used as anti-infectives was screened in a 96-well microtitre assay for in vitro activity against M. mycetomatis employing resazurin viability assay [2].

The chloroform extract from roots of Haplophyllum tuberculatum (Forsskal) A. Juss. (Rutaceae) was found to be the most active. Activity in the extract was localized by HPLC-based activity profiling, and two lignans, nectandrin B (1) and tetrahydrofuroguaiacin B (2), were isolated from the active time window.

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Fig. 1
 

  • References

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