Planta Med 2019; 85(18): 1515
DOI: 10.1055/s-0039-3399964
Main Congress Poster
Poster Session 2
© Georg Thieme Verlag KG Stuttgart · New York

Antispasmodic phenolic compounds isolated from Morus nigra root bark

Z Zoofishan
2   Budapest University of Technology and Economics,, NMR Group, Department of Inorganic and Analytical Chemistry, Szt. Gellért Sq. 4, H-1111 Budapest, Hungary
,
N Kúsz
2   Budapest University of Technology and Economics,, NMR Group, Department of Inorganic and Analytical Chemistry, Szt. Gellért Sq. 4, H-1111 Budapest, Hungary
,
G Tóth
2   Budapest University of Technology and Economics,, NMR Group, Department of Inorganic and Analytical Chemistry, Szt. Gellért Sq. 4, H-1111 Budapest, Hungary
,
J Hajagos-Tóth
3   Department of Pharmacodynamics and Biopharmacy, University of Szeged,, H-6720 Szeged, Hungary
4   Department of Pharmacology and Pharmacotherapy, University of Szeged,, H-6720 Szeged, Hungary
,
A Kothencz
3   Department of Pharmacodynamics and Biopharmacy, University of Szeged,, H-6720 Szeged, Hungary
4   Department of Pharmacology and Pharmacotherapy, University of Szeged,, H-6720 Szeged, Hungary
,
R Gáspár
3   Department of Pharmacodynamics and Biopharmacy, University of Szeged,, H-6720 Szeged, Hungary
4   Department of Pharmacology and Pharmacotherapy, University of Szeged,, H-6720 Szeged, Hungary
,
A Hunyadi
4   Department of Pharmacology and Pharmacotherapy, University of Szeged,, H-6720 Szeged, Hungary
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

Natural antispasmodic agents may have a potential for the treatment of asthma or gastrointestinal disorders including irritable bowel syndrome. Morus nigra has been valued as an antispasmodic traditional medicine but no related studies are available on its isolated constituents [1].

In this study, our aim was to investigate the antispasmodic potential of phenolic compounds of the root bark of M. nigra.

Seven compounds were isolated through the consecutive use of various preparative chromatographic techniques. These compounds were identified as morusin (1), kuwanon U (2), kuwanon E (3), moracin P (4), moracin O (5), albanol A (6), and albanol B (7). Their ex vivo antispasmodic activity was tested on isolated rat ileal and tracheal smooth muscles. Compound 2, 5 and 7 exerted strong activity on both models, while compound 3 (a methoxy analog of 2) was inactive. EC50 values and maximum effects for compounds 2 and 7 showed them equipotent with papaverin. Moracin O (5) exerted antispasmodic activity on ileal and tracheal muscles with EC50 values of 1.1 µM and 62 nM, respectively, and showed maximum effects on both muscles significantly higher than those of papavarine. Our findings suggest moracin O (5) as a new antispasmodic lead compound.

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Fig. 1
 

  • References

  • 1 Akhlaq A, Mehmood MH, Rehman A, Ashraf Z, Syed S, Bawany SA. etal. The Prokinetic, Laxative, and Antidiarrheal Effects of Morus nigra: Possible Muscarinic, Ca2+ Channel Blocking, and Antimuscarinic Mechanisms. Phytother Res 2016; 30: 1362-1376
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