Planta Med 2019; 85(18): 1512
DOI: 10.1055/s-0039-3399957
Main Congress Poster
Poster Session 2
© Georg Thieme Verlag KG Stuttgart · New York

Anti-inflammatory activity and ROS regulation of sinapaldehyde in LPS-stimulated RAW 264.7 macrophages

SH Baek
1   Department of Predictive Toxicology, Korea Institute of Toxicology,, Daejeon 34114, South Korea
2   Centre for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology,, Daejeon 34114, South Korea
,
MG Kang
1   Department of Predictive Toxicology, Korea Institute of Toxicology,, Daejeon 34114, South Korea
,
D Park
1   Department of Predictive Toxicology, Korea Institute of Toxicology,, Daejeon 34114, South Korea
2   Centre for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology,, Daejeon 34114, South Korea
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

Sinapaldehyde is a member of the class of compounds known as methoxyphenols. Sinapaldehyde can be found in plant extracts such as Senra incana and Ailanthus altissima Swingle [1],[2]. This study evaluated the anti-inflammatory effect of sinapaldehyde in LPS-stimulated RAW 264.7 macrophages using nitric oxide (NO) assay, cytokine ELISA, and reverse transcription polymerase chain reaction analyses. Sinapaldehyde exhibited inhibitory activity on the production of NO, ROS, and cytokines such as TNF-α and IL-6. Sinapaldehyde (100 μM) significantly inhibited total NO and ROS inhibitory activity at 93% (p < 0.001) and 34% (p < 0.05), respectively, when compared with LPS control. Moreover, sinapaldehyde significantly (p < 0.001) down-regulated the mRNA expression of inflammatory marker genes like TNF-α, IL-6, and iNOS against LPS stimulation. Sinapaldehyde did not show any cytotoxicity at different concentrations (3 - 100 µM). Our results demonstrate that sinapaldehyde is able to show anti-inflammatory effects by suppression of ROS and NO. It could be used as a new natural product for the effective treatment of inflammatory diseases.