Planta Med 2019; 85(18): 1491
DOI: 10.1055/s-0039-3399903
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

LC-MS guided the efficient isolation of afzelin and quercitrin from herbal plants

T-H Vo
1   The Ph.D. Program in Clinical Drug Development of Herbal Medicine, School of Pharmacy, Taipei Medical University,, 250 Wuxing St., 11031, Taipei Taiwan
,
C-K Lee
1   The Ph.D. Program in Clinical Drug Development of Herbal Medicine, School of Pharmacy, Taipei Medical University,, 250 Wuxing St., 11031, Taipei Taiwan
2   School of Pharmacy, Taipei Medical University,, 250 Wuxing St., 11031, Taipei Taiwan
,
Y-H Kuo
1   The Ph.D. Program in Clinical Drug Development of Herbal Medicine, School of Pharmacy, Taipei Medical University,, 250 Wuxing St., 11031, Taipei Taiwan
3   Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine,, 155-1, sec. 2, Linong St.,Taipei 112, Taiwan
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

Afzelin (AZ) and quercitrin (QC) are two available flavonoids found in some herbal medicine and show several promising bioactivities as antioxidant, antibacterial, anti-inflammation, and anti-tumor, etc [1]-[4]. We report here using LC-MS as guided direction to identify and isolate AZ and QC from ethanol extracts of six Vietnamese medicinal plants, Azadirachta indica (leaves)-AIL, Houttuynia cordata (aerial part)-HCA, Lophatherum gracile (aerial part)-LGA, Loranthus parasiticus (stem, leaves)-LPS, Oenanthe javanica (aerial part)-OJA, and Persea americana (leaves)-PAL.

Checking the standard samples of AZ and QC in LC-MS profiles, the ion peaks ([M-H]-) at m/z 431 and m/z 447 were determined to be AZ and QC, respectively. Other LC-MS profiles indicated that AIL, HCA, and PAL contained AZ and QC; whereas OJA and LPS only possessed afzelin, and LGA missed both AZ and QC. PAL (7 kg, dry) was further chosen to isolate AZ and QC due to the highest content. Extracted with ethanol 95%, then evaporated and partitioned with n-hexane and EtOAc sequently, the EtOAc layer (150 g) was yielded and further subjected to an open column with silica gel (n-hexane-EtOAc) to obtain four fractions. By LC-MS analyses, AZ and QC were found in fraction 2. Not only employing preparative HPLC but also using a C18 open column, the total amount of AZ (3.1 g) and QC (1.3 g) were yielded from fraction 2 (10 g). These results revealed that LC-MS analysis is a very useful method for providing the potent efficient isolation process to yield afzelin (AZ) and quercitrin (QC) from herbal plants.

 
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